Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties

• Published in: Marine drugs 2022-01, Vol.20 (1), p.60
• Main Authors: Krylova, Natalia V, Kravchenko, Anna O, Iunikhina, Olga V, Pott, Anastasia B, Likhatskaya, Galina N, Volod'ko, Aleksandra V, Zaporozhets, Tatyana S, Shchelkanov, Mikhail Y, Yermak, Irina M
• Format: Article
• Language: English
• Subjects: Algae; Analytical chemistry; Animals; Antiviral activity; Antiviral agents; Antiviral Agents - chemistry; Antiviral Agents - pharmacology; Aquatic Organisms; carrageenan; Carrageenan - chemistry; Carrageenan - pharmacology; Carrageenans; Cell interactions; Chlorocebus aethiops; Dengue fever; Disease resistance; Echoes; enterovirus; Enterovirus - drug effects; Enteroviruses; Glycoproteins; Herpes simplex; herpes simplex virus type 1; Herpesvirus 1, Human - drug effects; Humans; Molecular docking; Molecular Docking Simulation; Molecular weight; Physicochemical processes; Physicochemical properties; Polysaccharides; Rhodophyta; Saccharides; Severe acute respiratory syndrome coronavirus 2; Spectrum analysis; Structure-Activity Relationship; Vero cells; Vero Cells - drug effects; Viral infections; Viruses
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md20010060

The structural diversity and unique physicochemical properties of sulphated polysaccharides of red algae carrageenans (CRGs), to a great extent, determine the wide range of their antiviral properties. This work aimed to compare the antiviral activities of different structural types of CRGs: against herpes simplex virus type 1 (HSV-1) and enterovirus (ECHO-1). We found that CRGs significantly increased the resistance of Vero cells to virus infection (preventive effect), directly affected virus particles (virucidal effect), inhibited the attachment and penetration of virus to cells, and were more effective against HSV-1. CRG1 showed the highest virucidal effect on HSV-1 particles with a selective index (SI) of 100. CRG2 exhibited the highest antiviral activity by inhibiting HSV-1 and ECHO-1 plaque formation, with a SI of 110 and 59, respectively, when it was added before virus infection. CRG2 also significantly reduced the attachment of HSV-1 and ECHO-1 to cells compared to other CRGs. It was shown by molecular docking that tetrasaccharides-CRGs are able to bind with the HSV-1 surface glycoprotein, gD, to prevent virus-cell interactions. The revealed differences in the effect of CRGs on different stages of the lifecycle of the viruses are apparently related to the structural features of the investigated compounds.