Morpho-biochemical characterization of a RIL population for seed parameters and identification of candidate genes regulating seed size trait in lentil ( Lens culinaris Medik.)

• Published in: Frontiers in plant science 2023-02, Vol.14, p.1091432-1091432
• Main Authors: Dutta, Haragopal, K M, Shivaprasad, Aski, Muraleedhar S, Mishra, Gyan P, Sinha, Subodh Kumar, Vijay, Dunna, C T, Manjunath Prasad, Das, Shouvik, Pawar, Prashant Anupama-Mohan, Mishra, Dwijesh C, Singh, Amit Kumar, Kumar, Atul, Tripathi, Kuldeep, Kumar, Ranjeet Ranjan, Gupta, Sanjeev, Kumar, Shiv, Dikshit, Harsh Kumar
• Format: Article
• Language: English
• Subjects: BSA; cell membrane; cellulose; lignin; masur; Plant Science; videometer
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2023.1091432

The seed size and shape in lentil ( Medik.) are important quality traits as these influences the milled grain yield, cooking time, and market class of the grains. Linkage analysis was done for seed size in a RIL (F ) population derived by crossing L830 (20.9 g/1000 seeds) with L4602 (42.13 g/1000 seeds) which consisted of 188 lines (15.0 to 40.5 g/1000 seeds). Parental polymorphism survey using 394 SSRs identified 31 polymorphic primers, which were used for the bulked segregant analysis (BSA). Marker PBALC449 differentiated the parents and small seed size bulk only, whereas large seeded bulk or the individual plants constituting the large-seeded bulk could not be differentiated. Single plant analysis identified only six recombinant and 13 heterozygotes, of 93 small-seeded RILs (