Potential probiotic Lactobacillus delbrueckii subsp. lactis KUMS-Y33 suppresses adipogenesis and promotes osteogenesis in human adipose-derived mesenchymal stem cell

• Published in: Scientific reports 2024-04, Vol.14 (1), p.9689-9689, Article 9689
• Main Authors: Goudarzi, Farjam, Kiani, Amir, Nami, Yousef, Shahmohammadi, Azin, Mohammadalipour, Adel, Karami, Afshin, Haghshenas, Babak
• Format: Article
• Language: English
• Subjects: 631/326; 631/61; 631/80; Adipocytes; Adipocytes - cytology; Adipocytes - drug effects; Adipocytes - metabolism; Adipogenesis; Adipogenesis - drug effects; Adipose Tissue - cytology; Adipose Tissue - metabolism; Alkaline phosphatase; Cbfa-1 protein; CCAAT/enhancer-binding protein; Cell Differentiation - drug effects; Cells, Cultured; Collagen (type I); Fibroblast growth factor 23; Gene expression; Human Adipose-derived stem cells; Humanities and Social Sciences; Humans; Lactobacillus; Lactobacillus delbrueckii; Lactobacillus delbrueckii - metabolism; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; Microorganisms; multidisciplinary; Osteocalcin; Osteocytes; Osteogenesis; Osteogenesis - drug effects; Osteoporosis; Prevention; Probiotics; Probiotics - pharmacology; Science; Science (multidisciplinary); Stem cells
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-60061-2

Today, probiotics are considered to be living microorganisms whose consumption has a certain number of beneficial effects on the consumer. The present study aimed to investigate the effect of a new probiotic extract ( Lactobacillus delbrueckii subsp. lactis KUMS Y33) on the differentiation process of human adipose-derived stem cells (hADSCs) into adipocytes and osteocytes and, as a result, clarify its role in the prevention and treatment of bone age disease. Several bacteria were isolated from traditional yogurt. They were evaluated to characterize the probiotic’s activity. Then, the isolated hADSCs were treated with the probiotic extract, and then osteogenesis and adipogenesis were induced. To evaluate the differentiation process, oil red O and alizarin red staining, a triglyceride content assay, an alkaline phosphatase (ALP) activity assay, as well as real-time PCR and western blot analysis of osteocyte- and adipocyte-specific genes, were performed. Ultimately, the new strain was sequenced and registered on NBCI. In the probiotic-treated group, the triglyceride content and the gene expression and protein levels of C/EBP-α and PPAR-γ2 (adipocyte-specific markers) were significantly decreased compared to the control group ( P