Structural Proteomics of Herpesviruses

Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefor...

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Bibliographic Details
Published in:Viruses 2016-02, Vol.8 (2), p.50
Main Authors: Leroy, Baptiste, Gillet, Laurent, Vanderplasschen, Alain, Wattiez, Ruddy
Format: Article
Language:English
Subjects:
Animals
Herpesviridae - chemistry
Herpesviridae - genetics
Herpesviridae - metabolism
Herpesviridae Infections - virology
herpesvirus
host proteins
Humans
Life sciences
Mass Spectrometry
Microbiologie
Microbiology
proteomic
Proteomics
Review
Sciences du vivant
structural proteins
Viral Proteins - chemistry
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:Herpesviruses are highly prevalent viruses associated with numerous pathologies both in animal and human populations. Until now, most of the strategies used to prevent or to cure these infections have been unsuccessful because these viruses have developed numerous immune evasion mechanisms. Therefore, a better understanding of their complex lifecycle is needed. In particular, while the genome of numerous herpesviruses has been sequenced, the exact composition of virions remains unknown for most of them. Mass spectrometry has recently emerged as a central method and has permitted fundamental discoveries in virology. Here, we review mass spectrometry-based approaches that have recently allowed a better understanding of the composition of the herpesvirus virion. In particular, we describe strategies commonly used for proper sample preparation and fractionation to allow protein localization inside the particle but also to avoid contamination by nonstructural proteins. A collection of other important data regarding post-translational modifications or the relative abundance of structural proteins is also described. This review also discusses the poorly studied importance of host proteins in herpesvirus structural proteins and the necessity to develop a quantitative workflow to better understand the dynamics of the structural proteome. In the future, we hope that this collaborative effort will assist in the development of new strategies to fight these infections.
ISSN:1999-4915
1999-4915
DOI:10.3390/v8020050