Analysis of menstrual effluent: diagnostic potential for endometriosis

Endometriosis is a chronic and underdiagnosed disease which affects 5-10% of women of childbearing age and is characterized by growth of endometrial tissue outside of the uterus, most often in the peritoneal cavity. Delay in diagnosis is a major problem for management of this disorder, and treatment...

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Bibliographic Details
Published in:Molecular medicine (Cambridge, Mass.) Mass.), 2018-03, Vol.24 (1), p.1-1, Article 1
Main Authors: Warren, Laura A, Shih, Andrew, Renteira, Susana Marquez, Seckin, Tamer, Blau, Brandon, Simpfendorfer, Kim, Lee, Annette, Metz, Christine N, Gregersen, Peter K
Format: Article
Language:English
Subjects:
Adult
Antibodies
Antigens
Biomarkers
Biopsy
Birth control
Decidua
Decidualization
Effluents
Endometriosis
Endometriosis - diagnosis
Endometriosis - genetics
Female
Fibroblasts
Fibroblasts - metabolism
Flow cytometry
Gene Expression
Humans
Ischemia
Laparoscopy
Menstruation
Middle Aged
Phenotype
Software
Stromal fibroblast cells
Womens health
Young Adult
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Summary:Endometriosis is a chronic and underdiagnosed disease which affects 5-10% of women of childbearing age and is characterized by growth of endometrial tissue outside of the uterus, most often in the peritoneal cavity. Delay in diagnosis is a major problem for management of this disorder, and treatment is often not initiated until the disease has progressed for many years. Although the exact etiology of endometriosis remains unknown, retrograde menstruation is recognized as a common underlying factor leading to the deposit of menstrual effluent (ME) into the peritoneal cavity. Differences in the cellular biology and genetics of the cells within ME are therefore likely to explain why endometriosis develops in only a subset of women. Patients with and without endometriosis were consented to provide ME. ME was analyzed by flow cytometry for CD45- and CD45+ cell populations or used to isolate stromal fibroblast cells. ME-derived stromal fibroblast cells were assessed using decidualization assays following the addition of cAMP and IGFBP-1 concentrations in the culture supernatants were measured by ELISA. In addition, RNA was collected and analyzed by RNA-Seq and qPCR for markers of decidualization and to identify differentially expressed genes in ME-derived stromal fibroblast cells obtained from controls and subjects with endometriosis (±cAMP). Flow cytometry analysis of cell subsets within the CD45+ fraction of ME revealed a significant decrease in the number of uterine NK cells in endometriosis patients compared with controls (p 
ISSN:1076-1551
1528-3658
DOI:10.1186/s10020-018-0009-6