LC-MS determination and pharmacokinetic study of salidroside in rat plasma after oral administration of suspensions of traditional Chinese medicine Erzhi Wan and Fructus Ligustri lucidi

• Published in: Journal of pharmaceutical analysis 2011-02, Vol.1 (1), p.8-12
• Main Authors: Li, Ze-Yun, Li, Qing, Lü, Jiang, Ling, Jun-Hong, Yu, Xi-Hua, Chen, Xiao-Hui, Bi, Kai-Shun
• Format: Article
• Language: English
• Subjects: Erzhi Wan; Fructus Ligustri lucidi; liquid chromatography-mass spectrometry (LC-MS); Original article; pharmacokinetics; salidroside; 传统医药; 口服; 大鼠; 女贞子; 红景天苷; 药代动力学; 血浆
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/S2095-1779(11)70002-8

A simple,rapid and sensitive liquid chromatography-mass spectrometry（LC-MS）method was developed for the determination of salidroside in rat plasma and study of its pharmacokinetics after oral administration of suspension of Erzhi Wan and Fructus Ligustri lucidi into Wistar rats.Plasma sample of 200 μL was extracted with acetic ether-isopropanol（2∶1）and the extraction was performed on a Kromasil C18 column（150 mm×4.6 mm,5 μm）with the mobile phase of methanol-water（41∶59,v/v）within a run time of 6.0 min.The analyte was monitored with positive electrospray ionization（ESI）by selected ion monitoring（SIM）mode.The target ions were m/z 323.05 for salidroside and m/z 411.05 for internal standard（IS）geniposide.A good linear relationship was obtained over the range of 5.0-500.0 ng/mL and the lower limit of quantification was 5.0 ng/mL.The validated method was successfully applied to the pharmacokinetic study of salidroside in rat plasma after oral administration of suspension of Erzhi Wan and Fructus Ligustri lucidi.