Passive fluidic chip composed of integrated vertical capillary tubes developed for on-site SPR immunoassay analysis targeting real samples

We have successfully developed a surface plasmon resonance (SPR) measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W) × 160 mm(D) × 120 mm(H)) and a microfluidic immunoassay chip (16 mm(W) × 16 mm(D) × 4 mm(H)) that needs no e...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2012-06, Vol.12 (6), p.7095-7108
Main Authors: Horiuchi, Tsutomu, Miura, Toru, Iwasaki, Yuzuru, Seyama, Michiko, Inoue, Suzuyo, Takahashi, Jun-ichi, Haga, Tsuneyuki, Tamechika, Emi
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antigens
Antigens - analysis
Bands
Binding
Blood vessels
Calibration
Chips
Electrons
Immunoassay
Immunoassay - instrumentation
Immunoglobulin G - analysis
Ligands
Linear Models
Microfluidics
Microfluidics - instrumentation
Milk
Milk - chemistry
on-site
Onsite
passive pump
Pumps
raw sample
Rheology
Sensors
SPR
Surface Plasmon Resonance - instrumentation
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Summary:We have successfully developed a surface plasmon resonance (SPR) measurement system for the on-site immunoassay of real samples. The system is composed of a portable SPR instrument (290 mm(W) × 160 mm(D) × 120 mm(H)) and a microfluidic immunoassay chip (16 mm(W) × 16 mm(D) × 4 mm(H)) that needs no external pump system. An integrated vertical capillary tube functions as a large volume (150 μL) passive pump and a waste reservoir that has sufficient capacity for several refill operations. An immunoassay was carried out that employed the direct injection of a buffer and a test sample in sequence into a microfluidic chip that included 9 antibody bands and 10 reference reagent bands immobilized in the flow channel. By subtracting a reliable averaged reference sensorgram from the antibody, we effectively reduced the influence of the non-specific binding, and then our chip successfully detected the specific binding of spiked IgG in non-homogeneous milk. IgG is a model antigen that is certain not to be present in non-homogeneous milk, and non-homogeneous milk is a model of real sample that includes many interfering foreign substances that induce non-specific binding. The direct injection of a real sample with no pretreatment enabled us to complete the entire immunoassay in several minutes. This ease of operation and short measuring time are acceptable for on-site agricultural, environmental and medical testing.
ISSN:1424-8220
1424-8220
DOI:10.3390/s120607095