Evaluation of the Influence of Hypolipidemic Medication on Albino Wistar Rats' Bone Tissue by NMR Diffusiometry

• Published in: Medicina (Kaunas, Lithuania) Lithuania), 2024-06, Vol.60 (6), p.918
• Main Authors: Orban, Emese, Pap, Zsuzsanna, Fechete, Radu, Sipos, Remus Sebastian
• Format: Article
• Language: English
• Subjects: Albinism; Animals; Apolipoproteins; Atherosclerosis; Bone and Bones - drug effects; Bone density; Bone marrow; Cardiovascular disease; Cholesterol; Disease Models, Animal; Female; Females; Femur - drug effects; Fenofibrate; Fenofibrate - pharmacology; Fenofibrate - therapeutic use; Heart diseases; High density lipoprotein; Homeostasis; Hypolipidemic Agents - administration & dosage; Hypolipidemic Agents - pharmacology; Hypolipidemic Agents - therapeutic use; hypolipidemic medication; Laboratory animals; Lipids; Low density lipoprotein; Magnetic Resonance Spectroscopy - methods; menopause; Metabolic disorders; NMR; NMR diffusiometry; Nuclear magnetic resonance; Oophorectomy; Osteoporosis; Ovariectomy; Porous materials; Rats; Rats, Wistar; Risk factors; Statins
• ISSN: 1648-9144; 1010-660X; 1648-9144
• DOI: 10.3390/medicina60060918

The ongoing concern of the medical profession regarding chronic medication is related to increasing patient adherence and compliance to treatment and reducing medication side effects. In this respect, drugs represented by fixed-dose combinations of active substances within the same tablet have emerged. Such a principle can be extrapolated by following the potential beneficial effects that a chronic medication can have on chronic pathologies affecting different systems. The study included 48 female Albino Wistar rats, aged 16-18 months, which were divided into two groups: ovariectomized and non-ovariectomized rats. One batch of 12 non-ovariectomized rats received no treatment, becoming a control batch (NOVX-M). The ovariectomized (OVX) group was divided into 3 batches of 12 rats each: no treatment, control (OVX-M), fenofibrate-treated (OVX-F) and statin-treated (OVX-S) rats. At 12 weeks after ovariectomy, a femoral fracture occurred in the right hind limb of all animals included in the experiment To reveal the changes, at intervals of 2, 4, 6 and 8 weeks post-fracture, the proximal part of the femur was evaluated by NMR diffusiometry, which allows random motion of proton molecules expressed by self-diffusion coefficients, , thus allowing analysis of the size and complexity of microscopic order cavities within biological structures, such as pores inside bones. The effects of hypolipidemic medication in the absence of estrogen were evidenced, proving the beneficial effect that fenofibrate can have in preserving healthy tissue exposed to osteoporotic risk during the menopausal period. The effects of lipid-lowering medication are also influenced by the duration of administration. Osteoporosis and heart disease are two chronic pathologies that affect mainly female population in the second half of life, and proving the dual therapeutic potential of lipid-lowering medication may also have positive effects by increasing adherence and compliance to treatment.