Disturbance of Key Cellular Subproteomes upon Propofol Treatment Is Associated with Increased Permeability of the Blood-Brain Barrier

Background: Propofol is a short-acting anesthetic, which is often used for induction and maintenance of general anesthesia, sedation for mechanically ventilated adults and procedural sedation. Several side effects of propofol are known and a substantial number of patients suffer from post-operative...

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Bibliographic Details
Published in:Proteomes 2022-08, Vol.10 (3), p.28
Main Authors: Längrich, Timo, Bork, Kaya, Horstkorte, Rüdiger, Weber, Veronika, Hofmann, Britt, Fuszard, Matt, Olzscha, Heidi
Format: Article
Language:English
Subjects:
Alzheimer's disease
Analysis
Anesthesia
anesthetics
Blood-brain barrier
Brain
Cell culture
DNA damage
DNA damage response
drug effect
Edema
Endothelial cells
Fluorescein
human brain microvascular endothelial cells
Ischemia
Lipids
Mass spectrometry
Mass spectroscopy
Membrane permeability
metabolic stress
Metabolism
Metabolites
Microvasculature
Permeability
Propofol
Proteins
Proteomes
Proteomics
Sepsis
Side effects
Signal transduction
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Summary:Background: Propofol is a short-acting anesthetic, which is often used for induction and maintenance of general anesthesia, sedation for mechanically ventilated adults and procedural sedation. Several side effects of propofol are known and a substantial number of patients suffer from post-operative delirium after propofol application. In this study, we analyzed the effect of propofol on the function and protein expression profile on a proteome-wide scale. Methods: We cultured human brain microvascular endothelial cells in absence and presence of propofol and analyzed the permeability of the blood-brain barrier (BBB) by fluorescein passage and protein abundance on a proteome-wide scale by mass spectrometry. Results: Propofol interfered with the function of the blood-brain barrier. This was not due to decreased adhesion of propofol-treated human brain microvascular endothelial cells. The proteomic analysis revealed that some key pathways in these cells were disturbed, such as oxygen metabolism, DNA damage recognition and response to stress. Conclusions: Propofol has strong effects on protein expression which could explain several side effects of propofol.
ISSN:2227-7382
2227-7382
DOI:10.3390/proteomes10030028