In vitro Comparative Evaluation of Polylactic Acid/Polyglycolic Acid Copolymer and Expanded Polytetrafluoroethylene Based Guided Tissue Regeneration Membrane for Bacterial Colonisation

The study was designed to compare the extent of bacterial colonisation on the surface of Polylactic acid/Polyglycolic acid copolymer and expanded Polytetrafluoroethylene based Guided tissue regeneration (GTR) membrane in an in vitro model by spot analysis and scanning electron microscopy. Earlier in...

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Bibliographic Details
Published in:Journal of pure & applied microbiology : an international research journal of microbiology 2023-09, Vol.17 (3), p.1854-1862
Main Authors: Sareen, Shubham, Bey, Afshan, Zia, Afaf, Khan, Fatima
Format: Article
Language:English
Subjects:
bacterial adhesion
Comparative analysis
Electron microscopy
eptfe membrane
Fluorine compounds
guided tissue regeneration
pla/pga membrane
Polytetrafluoroethylene
scanning electron microscopy
spot test
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Summary:The study was designed to compare the extent of bacterial colonisation on the surface of Polylactic acid/Polyglycolic acid copolymer and expanded Polytetrafluoroethylene based Guided tissue regeneration (GTR) membrane in an in vitro model by spot analysis and scanning electron microscopy. Earlier in vitro models have aimed to study the barrier function against the bacterial penetration across just one surface of the GTR membranes. No such study is present in the literature which aimed to quantify bacterial adhesion over both the surfaces of the membrane. Sterile Resorbable PLA/PGA copolymer and non-resorbable microporous ePTFE based GTR membrane were used in the study. Both groups were subdivided into two subgroup (n=10) based on incubation period of 24 and 48 hours. Incubated in Todd Hewitt broth with Streptococcus mutans, the samples were vortexed and analysed for bacterial count using spot test and scanning electron microscopy. Between PLA/PGA and ePTFE GTR membrane at 24 hours incubation time period, there was a significant difference in terms of Colony forming units (p =
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.17.3.50