Lactobacillus paracasei subsp. paracasei 2004 improves health and lifespan in Caenorhabditis elegans

• Published in: Scientific reports 2024-05, Vol.14 (1), p.10453-10453, Article 10453
• Main Authors: Kishimoto, Saya, Nono, Masanori, Makizaki, Yutaka, Tanaka, Yoshiki, Ohno, Hiroshi, Nishida, Eisuke, Uno, Masaharu
• Format: Article
• Language: English
• Subjects: 631/208; 631/208/199; Acids; Aging; Animals; Bacteria; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - microbiology; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cellular stress response; E coli; Food; Food consumption; Forkhead protein; Forkhead Transcription Factors - genetics; Forkhead Transcription Factors - metabolism; Gastrointestinal Microbiome; Health; Humanities and Social Sciences; Hydrogen peroxide; Intestinal microflora; Lactic acid bacteria; Lactic acid bacterium (LAB); Lacticaseibacillus paracasei - genetics; Lacticaseibacillus paracasei - physiology; Life span; Longevity; Malnutrition; Microbiomes; Microbiota; Microorganisms; Middle age; multidisciplinary; Nematodes; Oxidative Stress; Probiotics; Science; Science (multidisciplinary); Supplements; The IIS pathway
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-60580-y

Recent research has highlighted the importance of the gut microbiome in regulating aging, and probiotics are interventions that can promote gut health. In this study, we surveyed several novel lactic acid bacteria to examine their beneficial effect on organismal health and lifespan in C. elegans . We found that animals fed some lactic acid bacteria, including L. acidophilus 1244 and L. paracasei subsp. paracasei 2004, grew healthy. Supplementation with the lactic acid bacterial strains L. acidophilus 1244 or L. paracasei subsp. paracasei 2004 significantly improved health, including food consumption, motility, and resistance to oxidative stressor, hydrogen peroxide. Our RNA-seq analysis showed that supplementation with L. paracasei subsp. paracasei 2004 significantly increased the expression of daf-16 , a C. elegans FoxO homolog, as well as genes related to the stress response. Furthermore, daf-16 deletion inhibited the longevity effect of L. paracasei subsp. paracasei 2004 supplementation. Our results suggest that L. paracasei subsp. paracasei 2004 improves health and lifespan in a DAF-16-dependent manner.