ANTIBACTERIAL PROPERTIES OF ZINC OXIDE NANOPARTICLES SYNTHESIZED BY THE SUPERNATANT OF WEISSELLA CONFUSA UPM22MT04

This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin-resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment...

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Bibliographic Details
Published in:Iraqi journal of agricultural science 2023-01, Vol.54 (5), p.1209-1222
Main Authors: Al-Tameemi, A I, Masarudin, M J, Rahim, R A, Timms, V, Neilan, B, Isa, N M
Format: Article
Language:English
Subjects:
Antibacterial agents
Antimicrobial agents
Bacteria
Biosynthesis
biosynthesis, Weissella confusa, ZnO-NPs, antibacterial activity, MRSA
Drug resistance
Fourier transforms
Infrared spectroscopy
Light scattering
Methicillin
Morphology
Nanoparticles
Nitrates
Particle size
Pathogens
Photon correlation spectroscopy
Physiology
Probiotics
Software
Spectrum analysis
Staphylococcus infections
Synthesis
Toxicity
Transmission electron microscopy
Wastewater treatment
Wastewater treatment plants
Water treatment
Weissella confusa
X-ray spectroscopy
Zinc oxide
Zinc oxides
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Summary:This study was aimed to produce zinc oxide nanoparticles (ZnO-NPs) using the supernatant of Weissella confusa UPM22MT04 and assess their effectiveness in inhibiting methicillin-resistant Staphylococcus aureus (MRSA). An isolate of Weissella confusa UPM22MT04 was isolated from a wastewater treatment plant in Johor, Malaysia, and was utilized to synthesize ZnO-NPs. The synthesized ZnO-NPs were characterized through several techniques, including UV-visible spectroscopy, Fourier-transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, and dynamic light scattering. Monodisperse spherical ZnO-NPs of 1.7 - 7.9 nm were obtained with 0.1 M zinc nitrate at 80°C. The biosynthesized ZnO-NPs exhibited vigorous inhibitory activity against MRSA. Results found that ZnO-NPs inhibited MRSA at a minimum concentration of 0.625 mg/mL and were bactericidal at a minimum concentration of 1.25 mg/mL. In MTT assays, ZnO-NPs showed no toxicity to HS-27 fibroblasts. The supernatant of Weissella confusa UPM22MT04 could be used to synthesize ZnO-NPs, which are an antibacterial agent, eco-friendly and non-toxic.
ISSN:0075-0530
2410-0862
DOI:10.36103/ijas.v54i5.1816