Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell....

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Bibliographic Details
Published in:Plant methods 2012-05, Vol.8 (1), p.15-15, Article 15
Main Authors: Chen, Zhong-Hua, Eisenach, Cornelia, Xu, Xin-Qin, Hills, Adrian, Blatt, Michael R
Format: Article
Language:English
Subjects:
Analysis
Arabidopsis
Arabidopsis thaliana
Cells
Cl- channel, Voltage-gated
Electrodes
Genetic resources
guard cells
Guards
homeostasis
K+ channel (voltage-gated)
leaves
Membrane conductance
Methodology
Microelectrode
Mutant analysis
Plant genetics
Plant physiology
potassium
quantitative analysis
Software utilities
tobacco
Vicia
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Description
Summary:Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K⁺ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.
ISSN:1746-4811
1746-4811
DOI:10.1186/1746-4811-8-15