Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis

Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell....

Full description

Saved in:
Bibliographic Details
Published in:Plant methods 2012-05, Vol.8 (1), p.15-15, Article 15
Main Authors: Chen, Zhong-Hua, Eisenach, Cornelia, Xu, Xin-Qin, Hills, Adrian, Blatt, Michael R
Format: Article
Language:English
Subjects:
Analysis
Arabidopsis
Arabidopsis thaliana
Cells
Cl- channel, Voltage-gated
Electrodes
Genetic resources
guard cells
Guards
homeostasis
K+ channel (voltage-gated)
leaves
Membrane conductance
Methodology
Microelectrode
Mutant analysis
Plant genetics
Plant physiology
potassium
quantitative analysis
Software utilities
tobacco
Vicia
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3
cites cdi_FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3
container_end_page 15
container_issue 1
container_start_page 15
container_title Plant methods
container_volume 8
creator Chen, Zhong-Hua
Eisenach, Cornelia
Xu, Xin-Qin
Hills, Adrian
Blatt, Michael R
description Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K⁺ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.
doi_str_mv 10.1186/1746-4811-8-15
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_7ae28f03a4b54e50a0b9a0890d926d42</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A534319361</galeid><doaj_id>oai_doaj_org_article_7ae28f03a4b54e50a0b9a0890d926d42</doaj_id><sourcerecordid>A534319361</sourcerecordid><originalsourceid>FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3</originalsourceid><addsrcrecordid>eNqFkk1v1DAQhiMEoqVw5QiRuMAhxR-xE3NAWlV8rFQJRKk4WhNnnLrKxls7Qey_x-mWpQtFJIdY48ePJvM6y55SckxpLV_TqpRFWVNa1AUV97LDXeH-rfVB9ijGS0JKyrh8mB0wJoSqaHmYffsc_OiN79_kfj26lYvY5tijGYNfX2yc733nDPQ5DNBvoou5t7kbRjBj3k0Q2txg38fcBr_KFwEa1_p1wh5nDyz0EZ_cfI-y8_fvvp58LE4_fVieLE6LpiJkLBAUWIPITCuJRaGUMFZJhYphzbhVbS0kSmGUsRUi5cBY1dSSKa5kxVt-lC233tbDpV4Ht4Kw0R6cvi740GkIozM96gqQ1ZZwKBtRoiBAGgWkVqRVTLYlS663W9d6albYGhzGAP2edH9ncBe68981LytBKpIEi62gcf4fgv0d41d6DknPIelaU5EcL2-aCP5qwjjqlMk8YxjQT1FTlR6hKJX_RynlqiaUzL_24g_00k8hRXpNsZLT1MBvqoM0LzdYn7o0s1QvBE-Q4nKmju-g0tviyhk_oHWpvnfg1d6BxIz4Y-xgilEvz77cKTfBxxjQ7qZHiZ5v_N_zenY7tB3-64on4PkWsOA1dMFFfX7GCC0JSeGXnPGfkP8DUw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1112431811</pqid></control><display><type>article</type><title>Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis</title><source>PMC (PubMed Central)</source><source>Publicly Available Content (ProQuest)</source><creator>Chen, Zhong-Hua ; Eisenach, Cornelia ; Xu, Xin-Qin ; Hills, Adrian ; Blatt, Michael R</creator><creatorcontrib>Chen, Zhong-Hua ; Eisenach, Cornelia ; Xu, Xin-Qin ; Hills, Adrian ; Blatt, Michael R</creatorcontrib><description>Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K⁺ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.</description><identifier>ISSN: 1746-4811</identifier><identifier>EISSN: 1746-4811</identifier><identifier>DOI: 10.1186/1746-4811-8-15</identifier><identifier>PMID: 22559714</identifier><language>eng</language><publisher>England: Springer-Verlag</publisher><subject>Analysis ; Arabidopsis ; Arabidopsis thaliana ; Cells ; Cl- channel, Voltage-gated ; Electrodes ; Genetic resources ; guard cells ; Guards ; homeostasis ; K+ channel (voltage-gated) ; leaves ; Membrane conductance ; Methodology ; Microelectrode ; Mutant analysis ; Plant genetics ; Plant physiology ; potassium ; quantitative analysis ; Software utilities ; tobacco ; Vicia</subject><ispartof>Plant methods, 2012-05, Vol.8 (1), p.15-15, Article 15</ispartof><rights>COPYRIGHT 2012 BioMed Central Ltd.</rights><rights>2012 Chen et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2012 Chen et al.; licensee BioMed Central Ltd. 2012 Chen et al.; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3</citedby><cites>FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475070/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1112431811?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22559714$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Zhong-Hua</creatorcontrib><creatorcontrib>Eisenach, Cornelia</creatorcontrib><creatorcontrib>Xu, Xin-Qin</creatorcontrib><creatorcontrib>Hills, Adrian</creatorcontrib><creatorcontrib>Blatt, Michael R</creatorcontrib><title>Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis</title><title>Plant methods</title><addtitle>Plant Methods</addtitle><description>Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K⁺ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.</description><subject>Analysis</subject><subject>Arabidopsis</subject><subject>Arabidopsis thaliana</subject><subject>Cells</subject><subject>Cl- channel, Voltage-gated</subject><subject>Electrodes</subject><subject>Genetic resources</subject><subject>guard cells</subject><subject>Guards</subject><subject>homeostasis</subject><subject>K+ channel (voltage-gated)</subject><subject>leaves</subject><subject>Membrane conductance</subject><subject>Methodology</subject><subject>Microelectrode</subject><subject>Mutant analysis</subject><subject>Plant genetics</subject><subject>Plant physiology</subject><subject>potassium</subject><subject>quantitative analysis</subject><subject>Software utilities</subject><subject>tobacco</subject><subject>Vicia</subject><issn>1746-4811</issn><issn>1746-4811</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqFkk1v1DAQhiMEoqVw5QiRuMAhxR-xE3NAWlV8rFQJRKk4WhNnnLrKxls7Qey_x-mWpQtFJIdY48ePJvM6y55SckxpLV_TqpRFWVNa1AUV97LDXeH-rfVB9ijGS0JKyrh8mB0wJoSqaHmYffsc_OiN79_kfj26lYvY5tijGYNfX2yc733nDPQ5DNBvoou5t7kbRjBj3k0Q2txg38fcBr_KFwEa1_p1wh5nDyz0EZ_cfI-y8_fvvp58LE4_fVieLE6LpiJkLBAUWIPITCuJRaGUMFZJhYphzbhVbS0kSmGUsRUi5cBY1dSSKa5kxVt-lC233tbDpV4Ht4Kw0R6cvi740GkIozM96gqQ1ZZwKBtRoiBAGgWkVqRVTLYlS663W9d6albYGhzGAP2edH9ncBe68981LytBKpIEi62gcf4fgv0d41d6DknPIelaU5EcL2-aCP5qwjjqlMk8YxjQT1FTlR6hKJX_RynlqiaUzL_24g_00k8hRXpNsZLT1MBvqoM0LzdYn7o0s1QvBE-Q4nKmju-g0tviyhk_oHWpvnfg1d6BxIz4Y-xgilEvz77cKTfBxxjQ7qZHiZ5v_N_zenY7tB3-64on4PkWsOA1dMFFfX7GCC0JSeGXnPGfkP8DUw</recordid><startdate>20120506</startdate><enddate>20120506</enddate><creator>Chen, Zhong-Hua</creator><creator>Eisenach, Cornelia</creator><creator>Xu, Xin-Qin</creator><creator>Hills, Adrian</creator><creator>Blatt, Michael R</creator><general>Springer-Verlag</general><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120506</creationdate><title>Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis</title><author>Chen, Zhong-Hua ; Eisenach, Cornelia ; Xu, Xin-Qin ; Hills, Adrian ; Blatt, Michael R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis</topic><topic>Arabidopsis</topic><topic>Arabidopsis thaliana</topic><topic>Cells</topic><topic>Cl- channel, Voltage-gated</topic><topic>Electrodes</topic><topic>Genetic resources</topic><topic>guard cells</topic><topic>Guards</topic><topic>homeostasis</topic><topic>K+ channel (voltage-gated)</topic><topic>leaves</topic><topic>Membrane conductance</topic><topic>Methodology</topic><topic>Microelectrode</topic><topic>Mutant analysis</topic><topic>Plant genetics</topic><topic>Plant physiology</topic><topic>potassium</topic><topic>quantitative analysis</topic><topic>Software utilities</topic><topic>tobacco</topic><topic>Vicia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Zhong-Hua</creatorcontrib><creatorcontrib>Eisenach, Cornelia</creatorcontrib><creatorcontrib>Xu, Xin-Qin</creatorcontrib><creatorcontrib>Hills, Adrian</creatorcontrib><creatorcontrib>Blatt, Michael R</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Plant methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Zhong-Hua</au><au>Eisenach, Cornelia</au><au>Xu, Xin-Qin</au><au>Hills, Adrian</au><au>Blatt, Michael R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis</atitle><jtitle>Plant methods</jtitle><addtitle>Plant Methods</addtitle><date>2012-05-06</date><risdate>2012</risdate><volume>8</volume><issue>1</issue><spage>15</spage><epage>15</epage><pages>15-15</pages><artnum>15</artnum><issn>1746-4811</issn><eissn>1746-4811</eissn><abstract>Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is not matched by accessible tools for quantitative analysis of transport in the intact cell. We have developed a reliable set of procedures for voltage clamp analysis of guard cells from Arabidopsis leaves. These procedures greatly simplify electrophysiological recordings, extending the duration of measurements and scope for analysis of the predominant K⁺ and anion channels of intact stomatal guard cells to that achieved previously in work with Vicia and tobacco guard cells.</abstract><cop>England</cop><pub>Springer-Verlag</pub><pmid>22559714</pmid><doi>10.1186/1746-4811-8-15</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1746-4811
ispartof Plant methods, 2012-05, Vol.8 (1), p.15-15, Article 15
issn 1746-4811
1746-4811
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_7ae28f03a4b54e50a0b9a0890d926d42
source PMC (PubMed Central); Publicly Available Content (ProQuest)
subjects Analysis
Arabidopsis
Arabidopsis thaliana
Cells
Cl- channel, Voltage-gated
Electrodes
Genetic resources
guard cells
Guards
homeostasis
K+ channel (voltage-gated)
leaves
Membrane conductance
Methodology
Microelectrode
Mutant analysis
Plant genetics
Plant physiology
potassium
quantitative analysis
Software utilities
tobacco
Vicia
title Protocol: optimised electrophyiological analysis of intact guard cells from Arabidopsis
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T04%3A14%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protocol:%20optimised%20electrophyiological%20analysis%20of%20intact%20guard%20cells%20from%20Arabidopsis&rft.jtitle=Plant%20methods&rft.au=Chen,%20Zhong-Hua&rft.date=2012-05-06&rft.volume=8&rft.issue=1&rft.spage=15&rft.epage=15&rft.pages=15-15&rft.artnum=15&rft.issn=1746-4811&rft.eissn=1746-4811&rft_id=info:doi/10.1186/1746-4811-8-15&rft_dat=%3Cgale_doaj_%3EA534319361%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b700t-ea9afcee2cd60fe5995cf969e92e823f9d856e65c9cf7ee13a227b862939673d3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1112431811&rft_id=info:pmid/22559714&rft_galeid=A534319361&rfr_iscdi=true