Distinct modes of telomere synthesis and extension contribute to Alternative Lengthening of Telomeres

• Published in: iScience 2024-01, Vol.27 (1), p.108655-108655, Article 108655
• Main Authors: Lu, Robert, Nelson, Christopher B., Rogers, Samuel, Cesare, Anthony J., Sobinoff, Alexander P., Pickett, Hilda A.
• Format: Article
• Language: English
• Subjects: Biological sciences; cell biology; molecular biology
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2023.108655

Alternative lengthening of telomeres (ALT) is a homology-directed repair mechanism that becomes activated in a subset of cancers to maintain telomere length. One of the defining features of ALT cells is the prevalence of extrachromosomal telomeric repeat (ECTR) DNA. Here, we identify that ALT cells engage in two modes of telomere synthesis. Non-productive telomere synthesis occurs during the G2 phase of the cell cycle and is characterized by newly synthesized internal telomeric regions that are not retained in the subsequent G1, coinciding with an induction of ECTR DNA. Productive telomere synthesis occurs specifically during the transition from G2 to mitosis and is defined as the extension of the telomere termini. While many proteins associated with break-induced telomere synthesis function in both non-productive and productive telomere synthesis, POLH specifically promotes productive telomere lengthening and suppresses non-productive telomere synthesis. These findings delineate the mechanism and cell cycle regulation of ALT-mediated telomere synthesis and extension. [Display omitted] •ALT cells engage in two modes of telomere synthesis•The bulk of ALT telomere synthesis takes place in G2 and is non-productive•Telomere extension predominantly occurs during the transition from G2 to mitosis Biological sciences; Molecular biology; Cell biology