Antibacterial and Biofilm Production Inhibition Activity of Thymus vulgaris L. Essential Oil against Salmonella spp. Isolates from Reptiles

Salmonellosis is an infectious disease affecting both animals and humans. Antimicrobial resistant (AMR) and biofilm-producing spp., frequently detected in reptiles (who can then act as asymptomatic carriers for warm-blooded animals), have developed resistance to biocides; this represents a warning f...

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Published in:Pathogens (Basel) 2023-06, Vol.12 (6), p.804
Main Authors: Galgano, Michela, Mrenoshki, Daniela, Pellegrini, Francesco, Capozzi, Loredana, Cordisco, Marco, Del Sambro, Laura, Trotta, Adriana, Camero, Michele, Tempesta, Maria, Buonavoglia, Domenico, Laricchiuta, Piero, Catella, Cristiana, Pratelli, Annamaria, Buonavoglia, Alessio, Corrente, Marialaura
Format: Article
Language:English
Subjects:
Animal diseases
Animals
antibiotic resistance
Antibiotics
Antimicrobial agents
Aqueous solutions
Bacteria
Bacteriology
Biocides
Biofilms
Biological activity
Cross-resistance
Dilution
Disinfectants
Essential oils
Genes
Homeotherms
Infectious diseases
Laboratories
Oils & fats
Pets
Public health
Reptiles
Reptiles & amphibians
Salmonella
Salmonellosis
Snakes
Thymus vulgaris
Veterinary medicine
Zoos
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Summary:Salmonellosis is an infectious disease affecting both animals and humans. Antimicrobial resistant (AMR) and biofilm-producing spp., frequently detected in reptiles (who can then act as asymptomatic carriers for warm-blooded animals), have developed resistance to biocides; this represents a warning for the emergence of biocide/antimicrobial cross-resistance. The aim of this study was to evaluate the efficacy of L. essential oil (TEO) in inhibiting bacterial growth and biofilm production of spp., which had been isolated from wild reptiles housed in a Zoo in Italy. The resistance profile against different classes of antibiotics showed that all the isolates were susceptible to the tested antibiotics, despite the presence of several AMR genes. All the isolates were also tested with aqueous solutions of TEO at different dilutions (5% to 0.039%). Interestingly, TEO proved effective both in inhibiting bacterial growth at low dilutions, with MIC and MBC values ranging between 0.078% and 0.312%, and in inhibiting biofilm production, with values ranging from 0.039% to 0.156%. TEO demonstrated effective bioactivity against the biofilm producer spp., proving to be a valid disinfectant for the prevention of salmonellosis from reptiles, a possible source of infection for humans exposed to the reptiles' environment.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens12060804