Response of Antarctic sea-ice algae to an experimental decrease in pH: a preliminary analysis from chlorophyll fluorescence imaging of melting ice

• Published in: Polar research 2018-01, Vol.37 (1), p.1438696-8
• Main Authors: Castrisios, Katerina, Martin, Andrew, Müller, Marius N., Kennedy, Fraser, McMinn, Andrew, Ryan, Ken G.
• Format: Article
• Language: English
• Subjects: Acidification; Algae; Antarctica; Biogeochemistry; Carbon dioxide; carbonate chemistry; Carbonates; Chemical analysis; Chemistry; Chlorophyll; Chlorophylls; Dynamics; Ecology; Fluorescence; Fluorimetry; Fluorometry; I-PAM: imaging pulse amplitude modulated fluorometry; Ice; Imaging; Imaging techniques; imaging-PAM; Marine biology; Melting; Melts; Microorganisms; Oceanography; Organic chemistry; PAR: photosynthetically active radiation; pH effects; photophysiology; Photosynthesis; Photosystem II; Physiology; Pulse amplitude; Salinity; Sea ice; Seawater; Speciation; Stress (physiology); UV-B: ultraviolet radiation; Water analysis; Water column
• ISSN: 1751-8369; 0800-0395; 1751-8369
• DOI: 10.1080/17518369.2018.1438696

Microorganisms confined to annual sea ice in the Southern Ocean are exposed to highly variable oxygen and carbonate chemistry dynamics because of the seasonal increase in biomass and limited exchange with the underlying water column. For sea-ice algae, physiological stress is likely to be exacerbated when the ice melts; however, variation in carbonate speciation has rarely been monitored during this important state-transition. Using pulse amplitude modulated fluorometry (Imaging-PAM, Walz), we documented in situ changes in the maximum quantum yield of photosystem II (F v /F m ) of sea-ice algae melting out into seawater with initial pH values ranging from 7.66 to 6.39. Although the process of ice-melt elevated seawater pH by 0.2-0.55 units, we observed a decrease in F v /F m between 0.02 and 0.06 for each unit drop in pH during real-time fluorescence imaging. These results are considered preliminary but provide context for including carbonate chemistry monitoring in the design of future sea ice state-transition experiments. Imaging-PAM is a reliable technology for determining F v /F m , but is of limited use for obtaining additional photosynthetic parameters when imaging melting ice.