An Early Block in the Replication of the Atypical Bluetongue Virus Serotype 26 in Culicoides Cells Is Determined by Its Capsid Proteins

Arboviruses such as bluetongue virus (BTV) replicate in arthropod vectors involved in their transmission between susceptible vertebrate-hosts. The "classical" BTV strains infect and replicate effectively in cells of their insect-vectors ( biting-midges), as well as in those of their mammal...

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Bibliographic Details
Published in:Viruses 2021-05, Vol.13 (5), p.919
Main Authors: Guimerà Busquets, Marc, Pullinger, Gillian D, Darpel, Karin E, Cooke, Lyndsay, Armstrong, Stuart, Simpson, Jennifer, Palmarini, Massimo, Fragkoudis, Rennos, Mertens, Peter P C
Format: Article
Language:English
Subjects:
Animals
arbovirus
Arthropods
atypical serotype
Biting
Bluetongue
Bluetongue virus - classification
Bluetongue virus - isolation & purification
Bluetongue virus - physiology
Bluetongue virus - ultrastructure
BTV-26
Capsid Proteins - metabolism
cell binding
Cell Line
Cells, Cultured
Culicoides
Disease transmission
Genomes
Host-Pathogen Interactions
Infections
Mammalian cells
Proteins
Replication
reverse genetics
RNA polymerase
Serogroup
Serotypes
Strains (organisms)
Vectors
Virus Attachment
Virus Replication - drug effects
Viruses
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Summary:Arboviruses such as bluetongue virus (BTV) replicate in arthropod vectors involved in their transmission between susceptible vertebrate-hosts. The "classical" BTV strains infect and replicate effectively in cells of their insect-vectors ( biting-midges), as well as in those of their mammalian-hosts (ruminants). However, in the last decade, some "atypical" BTV strains, belonging to additional serotypes (e.g., BTV-26), have been found to replicate efficiently only in mammalian cells, while their replication is severely restricted in cells. Importantly, there is evidence that these atypical BTV are transmitted by direct-contact between their mammalian hosts. Here, the viral determinants and mechanisms restricting viral replication in were investigated using a classical BTV-1, an "atypical" BTV-26 and a BTV-1/BTV-26 reassortant virus, derived by reverse genetics. Viruses containing the capsid of BTV-26 showed a reduced ability to attach to cells, blocking early steps of the replication cycle, while attachment and replication in mammalian cells was not restricted. The replication of BTV-26 was also severely reduced in other arthropod cells, derived from mosquitoes or ticks. The data presented identifies mechanisms and potential barriers to infection and transmission by the newly emerged "atypical" BTV strains in .
ISSN:1999-4915
1999-4915
DOI:10.3390/v13050919