Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene

The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on reti...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-07, Vol.22 (13), p.6732
Main Authors: Tabata, Kitako, Sugano, Eriko, Hatakeyama, Akito, Watanabe, Yoshito, Suzuki, Tomoya, Ozaki, Taku, Fukuda, Tomokazu, Tomita, Hiroshi
Format: Article
Language:English
Subjects:
Apoptosis
Cell death
channelrhodopsin
Exposure
Fluorescein
Fluorescence
Gene therapy
Genes
Light
light damage
Light intensity
Luminous intensity
Photoreceptors
Photosensitivity
Phototoxicity
Retina
Retinal ganglion cells
retinitis pigmentosa
Transduction
Visual evoked potentials
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Summary:The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on retinal ganglion cells after transduction of the optogenetic gene mVChR1 using an adeno-associated virus vector. Rats were exposed to continuous light for a week, and visually evoked potentials (VEPs) were recorded. The intensities of continuous light (500, 1000, 3000, and 5000 lx) increased substantially after VEP recordings. After the final recording of VEPs, retinal ganglion cells (RGCs) were retrogradely labeled with a fluorescein tracer, FluoroGold, and the number of retinal ganglion cells was counted under a fluorescent microscope. There was no significant reduction in the amplitudes of VEPs and the number of RGCs after exposure to any light intensity. These results indicated that RGCs were photosensitive after the transduction of optogenetic genes and did not induce any phototoxicity by continuous light exposure.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms22136732