Non-Specific Adsorption Reduction Methods in Biosensing

Non-specific adsorption (NSA) is a persistent problem that negatively affects biosensors, decreasing sensitivity, specificity, and reproducibility. Passive and active removal methods exist to remedy this issue, by coating the surface or generating surface forces to shear away weakly adhered biomolec...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2019-05, Vol.19 (11), p.2488
Main Authors: Lichtenberg, Jessanne Y, Ling, Yue, Kim, Seunghyun
Format: Article
Language:English
Subjects:
Adsorption
biofouling
Biomarkers
Biomolecules
Biosensors
Computer engineering
Enzymes
Fourier transforms
Glucose
Immunoglobulins
Methods
non-specific adsorption
Proteins
Review
Reynolds number
Sensors
Surface acoustic waves
Surface chemistry
surface functionalization
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Summary:Non-specific adsorption (NSA) is a persistent problem that negatively affects biosensors, decreasing sensitivity, specificity, and reproducibility. Passive and active removal methods exist to remedy this issue, by coating the surface or generating surface forces to shear away weakly adhered biomolecules, respectively. However, many surface coatings are not compatible or effective for sensing, and thus active removal methods have been developed to combat this phenomenon. This review aims to provide an overview of methods of NSA reduction in biosensing, focusing on the shift from passive methods to active methods in the past decade. Attention is focused on protein NSA, due to their common use in biosensing for biomarker diagnostics. To our knowledge, this is the first review to comprehensively discuss active NSA removal methods. Lastly, the challenges and future perspectives of NSA reduction in biosensing are discussed.
ISSN:1424-8220
1424-8220
DOI:10.3390/s19112488