Polygalaxanthone III downregulates inflammation in the lipopolysaccharide-stimulated RAW264.7 macrophages: A quantibody array analysis

Polygala japonica Houtt. (PJ), a member of the Polygala L. family that is suggested to exhibit detoxification properties in traditional Chinese medicine, is often used to treat upper respiratory tract infections. The anti-inflammatory effects of four main components of PJ (POL, PS-XLIX, PS-E, and PS...

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Bibliographic Details
Published in:Journal of pharmacological sciences 2021-10, Vol.147 (2), p.184-191
Main Authors: Wu, Yinan, Fu, Hongwei, Yang, Xiaobin, Leng, Fang, Huang, Yadong, Deng, Hong, Xiang, Qi, Zhang, Shu
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents
Cytokines - genetics
Cytokines - metabolism
Down-Regulation - drug effects
Drugs, Chinese Herbal - pharmacology
Gene Expression - drug effects
Glycosides - pharmacology
IL21
Inflammation - etiology
Inflammation - genetics
Inflammation Mediators - metabolism
Inflammation modulation
Interleukins - genetics
Interleukins - metabolism
Lipopolysaccharides - adverse effects
Mice
Nitric Oxide - genetics
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - genetics
Nitric Oxide Synthase Type II - metabolism
Polygala - chemistry
Polygala japonica Houtt
Polygalaxanthone III
Quantibody array
RAW 264.7 Cells
Reactive Oxygen Species - metabolism
Signal Transduction - drug effects
Xanthones - pharmacology
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Summary:Polygala japonica Houtt. (PJ), a member of the Polygala L. family that is suggested to exhibit detoxification properties in traditional Chinese medicine, is often used to treat upper respiratory tract infections. The anti-inflammatory effects of four main components of PJ (POL, PS-XLIX, PS-E, and PS-F) were examined using the LPS(0.3 μg·mL−1)-stimulated RAW264.7 macrophage model. The levels of NO, ROS, and iNOS were examined to analyze the anti-inflammatory activity of POL. Additionally, the levels of extracellular inflammation-related cytokines and chemokines were measured using quantibody array. The KEGG pathway analysis was performed to examine the anti-inflammatory mechanism of POL. The levels of NO in the POL-pretreated group were significantly downregulated when compared with those in the PS-E-pretreated, PS-F-pretreated, and PS-XLIX-pretreated groups. POL significantly inhibited the changes of iNOS, ROS, and inflammatory factors caused by LPS stimulation (p 
ISSN:1347-8613
1347-8648
DOI:10.1016/j.jphs.2021.06.010