Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreacto...

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Bibliographic Details
Published in:Plants (Basel) 2021-05, Vol.10 (5), p.1010
Main Authors: Mancilla-Álvarez, Eucario, Pérez-Sato, Juan Antonio, Núñez-Pastrana, Rosalía, Spinoso-Castillo, José L., Bello-Bello, Jericó J.
Format: Article
Language:English
Subjects:
Acclimatization
Automation
Biofuels
Bioreactors
Chlorophyll
Culture media
Design
Efficiency
Ethanol
in vitro multiplication
Micropropagation
Multiplication
Physiology
Propagation
semi-automated bioreactors
Semisolids
Shoots
Stomata
stomatal content
Survival
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Summary:Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants10051010