Development and Application of an In Vitro Method to Evaluate Anthracnose Resistance in Soybean Germplasm

Anthracnose caused by is a major fungal disease of soybean, especially vegetable soybean (edamame). Studies of this disease have mainly focused on resistance evaluation, but the primary methods used-in vivo inoculation of pods or plants under greenhouse or field conditions-have limitations with resp...

Full description

Saved in:
Bibliographic Details
Published in:Plants (Basel) 2022-02, Vol.11 (5), p.657
Main Authors: Zhu, Longming, Feng, Lele, Yu, Xiaomin, Fu, Xujun, Yang, Qinghua, Jin, Hangxia, Yuan, Fengjie
Format: Article
Language:English
Subjects:
Anthracnose
Colletotrichum truncatum
Cultivars
Efficiency
Flowering
Fungal diseases
Germplasm
In vitro methods and tests
In vivo methods and tests
Infections
Inoculation
Molecular modelling
Mycelia
Normal distribution
Pathogens
Plant breeding
resistance evaluation
Seeds
soybean
Soybeans
Spraying
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Anthracnose caused by is a major fungal disease of soybean, especially vegetable soybean (edamame). Studies of this disease have mainly focused on resistance evaluation, but the primary methods used-in vivo inoculation of pods or plants under greenhouse or field conditions-have limitations with respect to accuracy, stability, scale, and environmental safety. In this study, we developed a method for inoculating pods in vitro by soaking in a mycelial suspension. We optimized the crucial components, including the mycelial suspension concentration (40 to 60 mg mL ), the maturity of the sampled pods (15 days after flowering), and the post-inoculation incubation period (5 days). Application of the mycelial suspension by soaking rather than spraying improved the efficiency of inoculation and made large-scale evaluation possible. Using this method, we evaluated 589 soybean germplasm resources (275 cultivars, 233 landraces, and 81 wild accessions). We identified 25 highly resistant cultivars, 11 highly resistant landraces, but only one highly resistant wild accession. Our results will aid future research on soybean anthracnose resistance, including gene discovery, the elucidation of molecular mechanisms, and the breeding of resistant cultivars.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11050657