Prognostic Significance of the Microbiome and Stromal Cells Phenotype in Esophagus Squamous Cell Carcinoma

Esophageal cancer is one of the most aggressive malignant neoplasms, with low survival rates and limited treatment options. In this study we analyzed the microbiome composition and the phenotype of inflammatory tumor infiltrate in squamous cell carcinoma of esophagus (ESCC) and examined possible rel...

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Bibliographic Details
Published in:Biomedicines 2021-06, Vol.9 (7), p.743
Main Authors: Kovaleva, Olga, Podlesnaya, Polina, Rashidova, Madina, Samoilova, Daria, Petrenko, Anatoly, Mochalnikova, Valeria, Kataev, Vladimir, Khlopko, Yuri, Plotnikov, Andrey, Gratchev, Alexei
Format: Article
Language:English
Subjects:
cancer
Cancer therapies
Cloning
Esophageal cancer
Esophagus
Genotype & phenotype
Gram-positive bacteria
Immunotherapy
Inflammation
macrophage
Macrophages
Medical prognosis
Medical research
Metastasis
microbiome
Microbiomes
Neoplasia
Nitric-oxide synthase
Phenotypes
prognosis
Squamous cell carcinoma
stroma
Stromal cells
Surgery
Tumor cells
Tumors
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Summary:Esophageal cancer is one of the most aggressive malignant neoplasms, with low survival rates and limited treatment options. In this study we analyzed the microbiome composition and the phenotype of inflammatory tumor infiltrate in squamous cell carcinoma of esophagus (ESCC) and examined possible relationships between them and their prognostic significance. We found that the predominant phyla of microorganisms found in both tumors and adjacent normal tissues were Firmicutes, Proteobacteria, Actinobacteria, Gemmatimonadetes and Bacteroidetes. We established that only bacteria of the genus Staphylococcus differ between tumors and normal tissues. We found a significant correlation between bacterial burden and the phenotype of the tumor stroma. Namely, a group of tumors characterized by a high expression of CD206 (r = −0.3976, p = 0.0056) in the stroma and iNOS (r = −0.2953, p = 0.0439) in tumor cells is characterized by a higher bacterial burden. Further, we established that in the group with a high content of CD206+ macrophages, there is also a predominance of gram-positive bacteria over gram-negative ones. We found that gram-positive bacterial burden is associated with disease prognosis in ESCC showing high content of CD206+ macrophages. In conclusion we established that the tumor microbiome, can be prognostically significant for ESCC when combined with other stromal markers.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines9070743