Investigation of the effect of P14 promoter aberrant methylation on the biological function of human lung cancer cells

Background This study was conducted to investigate the effect of P14 promoter aberrant methylation on the biological function of human lung adenocarcinoma cells. Methods We used nested methylation‐specific PCR (NMSP) to detect the methylation status of the p14ARF promoter region in SPCA1 and BEAS2B...

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Bibliographic Details
Published in:Thoracic cancer 2019-06, Vol.10 (6), p.1388-1394
Main Authors: Jia, Bing‐Yang, Yang, Rong‐Hua, Jiao, Wen‐Jie, Tian, Kai‐Hua
Format: Article
Language:English
Subjects:
Adenocarcinoma of lung
Apoptosis
Cancer cells
DNA methylation
Investigations
Lung cancer
Messenger RNA
Methylation
Original
p14ARF promoter regions
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Summary:Background This study was conducted to investigate the effect of P14 promoter aberrant methylation on the biological function of human lung adenocarcinoma cells. Methods We used nested methylation‐specific PCR (NMSP) to detect the methylation status of the p14ARF promoter region in SPCA1 and BEAS2B cell lines. The experimental groups were treated with 5‐aza‐2′‐deoxycytidine (5‐Aza). Quantitative real‐time PCR, Western blot, flow cytometry, and Cell Counting Kit 8 were used to detect the expression of p14ARF messenger RNA and protein in each group, apoptosis, and cell proliferation inhibition, respectively. Results NMSP detected that the p14 promoter region of SPCA1 cells has abnormal methylation status. After treatment with 5‐Aza, the expression of p14ARF messenger RNA and protein in SPCA1 cells (P 
ISSN:1759-7706
1759-7714
DOI:10.1111/1759-7714.13082