Psoralen Inactivation of Viruses: A Process for the Safe Manipulation of Viral Antigen and Nucleic Acid

High consequence human pathogenic viruses must be handled at biosafety level 2, 3 or 4 and must be rendered non-infectious before they can be utilized for molecular or immunological applications at lower biosafety levels. Here we evaluate psoralen-inactivated Arena-, Bunya-, Corona-, Filo-, Flavi- a...

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Bibliographic Details
Published in:Viruses 2015-11, Vol.7 (11), p.5875-5888
Main Authors: Schneider, Katherine, Wronka-Edwards, Loni, Leggett-Embrey, Melissa, Walker, Eric, Sun, Peifang, Ondov, Brian, Wyman, Travis H, Rosovitz, M J, Bohn, Sherry S, Burans, James, Kochel, Tadeusz
Format: Article
Language:English
Subjects:
Animals
antibody
Antigens
Antigens, Viral - immunology
Antiviral Agents - metabolism
biocontainment
biosafety
BSL-3
BSL-4
Cell culture
Cell death
Cell Line
Dengue fever
Ficusin - metabolism
Humans
IFA
Immunology
inactivation
Laboratories
Methods
Microbial Viability - drug effects
PCR
Phenols
psoralen
reverse transcription
RNA Viruses - drug effects
RNA Viruses - genetics
RNA Viruses - immunology
RNA Viruses - physiology
RNA, Viral - genetics
safety
sequencing
sterility
Toxicity
Trizol LS
virus
Virus Inactivation
Viruses
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Summary:High consequence human pathogenic viruses must be handled at biosafety level 2, 3 or 4 and must be rendered non-infectious before they can be utilized for molecular or immunological applications at lower biosafety levels. Here we evaluate psoralen-inactivated Arena-, Bunya-, Corona-, Filo-, Flavi- and Orthomyxoviruses for their suitability as antigen in immunological processes and as template for reverse transcription PCR and sequencing. The method of virus inactivation using a psoralen molecule appears to have broad applicability to RNA viruses and to leave both the particle and RNA of the treated virus intact, while rendering the virus non-infectious.
ISSN:1999-4915
1999-4915
DOI:10.3390/v7112912