Structure and expression identification of Cherry Valley duck IRF8

• Published in: Poultry science 2022-02, Vol.101 (2), p.101598-101598, Article 101598
• Main Authors: Zhang, Tingting, Zhai, Xinyu, Wang, Xiuyuan, Wang, Jinchao, Xing, Bin, Miao, Runchun, Li, Tianxu, Hong, Tianqi, Wei, Liangmeng
• Format: Article
• Language: English
• Subjects: Animals; Cherry Valley duck; Chickens; cloning; Cloning, Molecular; Immunity, Innate - genetics; IMMUNOLOGY, HEALTH AND DISEASE; innate immunity; Interferon Regulatory Factors - genetics; IRF8; Phylogeny; viral infection
• ISSN: 0032-5791; 1525-3171
• DOI: 10.1016/j.psj.2021.101598

Interferon regulatory factor 8 (IRF8) is also known as interferon (IFN) consensus sequence binding protein (ICSBP), which plays an important role in IFN signal transduction. In this study, we cloned the full-length coding sequence of Cherry Valley duck IRF8 (duIRF8) and analyzed its structure. In addition, we tested the distribution of IRF8 in the tissues of healthy Cherry Valley ducks, and the changes in IRF8 expression levels in the tissues after virus infection. The results show that the open reading frame (ORF) of IRF8 is 1293 bp, encodes 430 amino acids, and have 3 conserved domains: the N-terminal DBD domain, the C-terminal IAD domain, and the NLS domain. Besides, from the analysis of the phylogenetic tree, it can be known that the duIRF8 has the highest homology with the anser cygnoides, and has less homology with the fish. Analyzing the distribution level of IRF8 in the tissues, it is found that the expression level of IRF8 in the liver of Cherry Valley duck is the highest. However, after infection with duck Tambusu virus, novel duck reovirus, and duck plague virus, the expression of IRF8 in the spleen and brain all showed up-regulation. These data indicate that IRF8 is involved in the host's innate immune response against virus in Cherry Valley duck.