Effects of Saffron (Crocus sativus L.) Aqueous Extract on In vitro Maturation, Fertilization and Embryo Development of Mouse Oocytes

Lower pregnancy rates of in vitro matured oocytes compared to those of in vivo stimulated cycles indicate that optimization of in vitro maturation (IVM) remains a challenge. Reduced developmental competence of in vitro matured oocytes shows that current culture systems for oocyte maturation do not a...

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Bibliographic Details
Published in:Cell journal (Yakhteh) 2012, Vol.13 (4), p.259-264
Main Authors: Tavana, Somayeh, Eimani, Hussein, Azarnia, Mahnaz, Shahverdi, Abdolhossein, Eftekhari-Yazdi, Poopak
Format: Article
Language:English
Subjects:
Antioxidant
Crocus sativus
In vitro maturation
Mice
Oocyte
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Summary:Lower pregnancy rates of in vitro matured oocytes compared to those of in vivo stimulated cycles indicate that optimization of in vitro maturation (IVM) remains a challenge. Reduced developmental competence of in vitro matured oocytes shows that current culture systems for oocyte maturation do not adequately support nuclear and/or cytoplasmic maturation. Therefore this study evaluates the effects of different concentrations of saffron (Crocus sativus L.) aqueous extract (SAE), as an antioxidant agent on IVM of immature mouse oocytes. In this experimental study ,cumulus-oocyte complexes (COCs) were collected from 6-8 weeks old novel medical research institute (NMRI) female mice ovaries. COCs were cultured in IVM medium supplemented with 0 (control), 5, 10, 20 and 40 µg/ml of SAE in 5% CO2 at 37℃. The rates of maturation, fertilization and development were recorded. ANOVA and Duncan's protected least significant test, using the SAS program was applied for all statistical analysis. The maturation rate was significantly higher in all groups treated with different concentrations of SAE compared with the control group (p
ISSN:2228-5806
2228-5814