Effects of berberine hydrochloride on immune response in the crab Charybdis japonica

• Published in: BMC genomics 2022-08, Vol.23 (1), p.1-578, Article 578
• Main Authors: Gao, Tian-heng, Han, Ming-ming, Zhou, Hui, Zhu, Chen-xi, Yang, Ying, Zuraini, Zakaria, Guo, Yan-Xia, Jiang, Qi-chen
• Format: Article
• Language: English
• Subjects: Adenylate cyclase; Bacterial infections; Berberine; berberine hydrochloride; Bioactive compounds; Biological products; Care and treatment; Caspase-8; Cathepsin L; Charybdis japonica; Complement activation; Crabs; Crustaceans; Enzymes; Experiments; Exposure; Fibrinogen; Gene expression; Genes; Genomics; Glycoproteins; Hepatopancreas; Herbal medicine; Immune response; Immune system; Infections; Kininogens; Metabolism; Molecular modelling; Neutralization; Pharmacology, Experimental; Phosphatase; Physiological aspects; Quality control; Salinity; Salts; Signal processing; Signal transduction; Survival; Testing; Transcriptome sequencing; Transcriptomes; Variance analysis; α2-Macroglobulin
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-022-08798-w

Berberine hydrochloride is the main effective component of Coptis spp. used in Chinese herbal medicine and its underlying molecular mechanisms, responsible for inducing effects in crustacean species, are not fully understood. In this study, the molecular response of the crab Charybdis japonica to berberine hydrochloride exposure was studied using transcriptome sequencing. The survival rate, gene expression and activities of several immune enzymes were measured after berberine hydrochloride treatments, with or without injection of the pathogenic bacterium Aeromonas hydrophila. A total of 962 differentially expressed genes (464 up-regulated and 498 down-regulated) were observed during exposure to 100 mg/L of berberine hydrochloride and in the control group after 48 h. Enrichment analysis revealed that these genes are involved in metabolism, cellular processes, signal transduction and immune functions, indicating that exposure to berberine hydrochloride activated the immune complement system. This bioactive compound simultaneously activated fibrinogen beta (FGB), fibrinogen alpha (FGA), alpha-2-macroglobulin (A2M), kininogen (KNG), fibrinogen gamma chain (FGB), alpha-2-HS-glycoprotein (AHSG), caspase-8 (CASP8), cathepsin L (CTSL), adenylate cyclase 3 (Adcy3) and MMP1. Its action could significantly increase the survival rate of the crabs injected with A. hydrophila and promote the activity of LZM, Caspas8, FGA, ACP and AKP in the hepatopancreas. When A. hydrophila was added, the neutralization of 300 mg/L berberine hydrochloride maximized the activities of Caspas8, LZM, ACP and AKP. Our results provide a new understanding of the potential effects of berberine hydrochloride on the immune system mechanisms in crustaceans.