Lacrimal secretory IgA in active posterior uveitis induced by Toxoplasma gondii

It is quite difficult to diagnose active toxoplasmosis in patients with ocular toxoplasmosis. Active posterior uveitis presumably due to Toxoplasma gondii infection (APUPT) is seldom produced during a prime-infection; hence most patients do not show high IgM antibodies. High levels of IgA have been...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2004-12, Vol.99 (8), p.861-864
Main Authors: Lynch, Maria Isabel, Cordeiro, Francisco, Ferreira, Silvana, Ximenes, Ricardo, Oréfice, Fernando, Malagueño, Elizabeth
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
anti-Toxoplasma gondii secretory IgA
antibodies anti-T. gondii
Antibodies, Protozoan - analysis
Biomarkers - analysis
Case-Control Studies
Child
Enzyme-Linked Immunosorbent Assay
Female
Fluorescent Antibody Technique, Indirect
Humans
Immunoglobulin A, Secretory - analysis
Immunoglobulin G - analysis
Male
ocular toxoplasmosis diagnosis
PARASITOLOGY
secretory IgA and ocular toxoplasmosis
Sensitivity and Specificity
Tears - immunology
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis, Ocular - diagnosis
TROPICAL MEDICINE
Uveitis, Posterior - parasitology
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Summary:It is quite difficult to diagnose active toxoplasmosis in patients with ocular toxoplasmosis. Active posterior uveitis presumably due to Toxoplasma gondii infection (APUPT) is seldom produced during a prime-infection; hence most patients do not show high IgM antibodies. High levels of IgA have been described in active toxoplasmosis. The purpose of this study was to investigate possible association between APUPT and the specific anti-parasite sIgA in tears. The study was carried out as case-control. Tears of 25 clinically confirmed APUPT patients and 50 healthy control subjects were analyzed. All were IgG seropositive. Specific sIgA was determined by ELISA assay using T. gondii RH strain crude extract. Anti-T. gondii sIgA was found in 84% of the cases and in 22% of the control subjects. The intensity of the reaction was higher in APUPT cases (P = 0.007). There was strong association between APUPT patients and lacrimal sIgA (odds-ratio 18.61, P = 0.0001). ELISA test sensitivity was 84% and specificity 78%. Our data suggest that anti-T.gondii secretory IgA found in tears may become an important marker for active ocular toxoplasmosis.
ISSN:0074-0276
1678-8060
0074-0276
1678-8060
DOI:10.1590/S0074-02762004000800013