Detection of exopolysaccharides (algD, pelF, and pslD) genes in burn wound Pseudomonas aeruginosa isolates

Background: Pseudomonas aeruginosa biofilm is an essential component of virulence that plays a significant role in antimicrobial resistance and chronic burn wound infections. Objective: This study aimed to investigate the biofilm formation capacity of P. aeruginosa isolated from chronic burn wound f...

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Bibliographic Details
Published in:Medical Journal of Babylon 2023-07, Vol.20 (3), p.595-599
Main Authors: Alwan, Zeena, Lilo, Rafal, Al-Taee, Zahraa, Mohsen, Liqaa, Al-Alaq, Farah
Format: Article
Language:English
Subjects:
biofilm
burn wounds
Burns and scalds
Care and treatment
Diagnosis
Drug resistance in microorganisms
exopolysaccharides
Genes
Genetic research
Health aspects
Infection
Medical research
Medicine, Experimental
Microbial mats
Patient outcomes
Pseudomonas aeruginosa
Pseudomonas aeruginosa infections
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Summary:Background: Pseudomonas aeruginosa biofilm is an essential component of virulence that plays a significant role in antimicrobial resistance and chronic burn wound infections. Objective: This study aimed to investigate the biofilm formation capacity of P. aeruginosa isolated from chronic burn wound from January to May 2022 by biochemical and molecular techniques. Materials and Methods: Quantification of biofilm was performed based on tube method for local isolates of P. aeruginosa after growing on brain heart Broth. The genes encoding exopolysaccharides (algD, pelF, and pslD) were targeted by using conventional polymerase chain reaction (PCR). Results: The results showed that 92.6% of isolates were biofilm former, interestingly 68% of isolates were considered as strong former comparing with other biofilm categories. Gel electrophoresis result of PCR products presented clear bands for algD and psID genes with percentages (96%) and (3.7%) respectively. However, there was no PCR product for pelf gene among all isolates. Conclusion: The prevalence of algD, the large operon necessary for alginate production, was high among P. aeruginosa biofilm producer in this study and it can be an essential agent in the pathogenicity of P. aeruginosa burn wound infections comparing with other biofilm genes (pelF and pslD) of exopolysaccharide structure.
ISSN:1812-156X
2312-6760
DOI:10.4103/MJBL.MJBL_534_23