Notch1 signaling is limited in healthy mature kidneys in vivo

A Delta-Notch signaling component, Notch1, is involved in the normal development and multiple disorders of the kidney. Although the increase in Notch1 signaling is crucial to these pathogeneses, the basal signaling level in 'healthy' mature kidneys is still unclear. To address this questio...

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Bibliographic Details
Published in:BMC research notes 2023-04, Vol.16 (1), p.54-54, Article 54
Main Authors: Sugiura, Ryosuke, Nakayama, Takahiro, Nishino, Teppei, Sambe, Naoto, Radtke, Freddy, Yoshihara, Masaharu, Takahashi, Satoru
Format: Article
Language:English
Subjects:
Animal models
Animals
Cells
Cre recombinase
Cre/loxP system
Delta-Notch signaling pathway
Diabetes
Disease model
Experiments
Fluorescence
Gal4/UAS system
Genetic engineering
Kidney - metabolism
Kidney diseases
Kidneys
Mice
Mice, Transgenic
Notch1 protein
Proteins
Receptor, Notch1 - genetics
Receptor, Notch1 - metabolism
Research Note
Signal Transduction
Transcription factors
Transgenic mice
Transgenic mouse
Tubules
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Summary:A Delta-Notch signaling component, Notch1, is involved in the normal development and multiple disorders of the kidney. Although the increase in Notch1 signaling is crucial to these pathogeneses, the basal signaling level in 'healthy' mature kidneys is still unclear. To address this question, we used an artificial Notch1 receptor fused with Gal4/UAS components in addition to the Cre/loxP system and fluorescent proteins in mice. This transgenic reporter mouse system enabled labeling of past and ongoing Notch1 signaling with tdsRed or Cre recombinase, respectively. We confirmed that our transgenic reporter mouse system mimicked the previously reported Notch1 signaling pattern. Using this successful system, we infrequently observed cells with ongoing Notch1 signaling only in Bowman's capsule and tubules. We consider that Notch1 activation in several lines of disease model mice was pathologically significant itself.
ISSN:1756-0500
1756-0500
DOI:10.1186/s13104-023-06326-x