Cytotoxic Evaluation and Determination of Organic and Inorganic Eluates from Restorative Materials

Over the last years, diverse commercial resin-based composites have dominated as dental filling materials. The purpose of the present study was to determine organic and inorganic eluates from five restorative materials using GC/MS and ICP–OES and to compare the effect on cell survival of human gingi...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-08, Vol.26 (16), p.4912
Main Authors: Roussou, Konstantina, Nikolaidis, Alexandros K., Ziouti, Fani, Arhakis, Aristidis, Arapostathis, Konstantinos, Koulaouzidou, Elisabeth A.
Format: Article
Language:English
Subjects:
Aluminum
Barium
Biocompatibility
Biological activity
Bisphenol A
Calcium
Cell culture
Cell viability
Chromatography
Composite materials
composite resins
Composition
Cytotoxicity
Deionization
Dental enamel
Dental materials
Dental restorative materials
Disks
Fibroblasts
Fluorides
gas chromatography
ion release
Ions
Light emitting diodes
Mass spectrometry
Molecular weight
Polymerization
Resins
Saliva
Scientific imaging
Solvents
Standard deviation
Sulforhodamine
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Summary:Over the last years, diverse commercial resin-based composites have dominated as dental filling materials. The purpose of the present study was to determine organic and inorganic eluates from five restorative materials using GC/MS and ICP–OES and to compare the effect on cell survival of human gingival fibroblasts of a conventional and a bioactive resin. Five commercially available restorative materials were employed for this study: ActivaTM Bioactive Restorative, ENA HRi, Enamel plus HRi Biofunction, Fuji II LC Capsule, and Fuji IX Capsule. Disks that were polymerized with a curing LED light or left to set were immersed in: 1 mL methanol or artificial saliva for GC/MS analysis, 5mL deionized water for ICP–OES, and 5mL of culture medium for cell viability. Cell viability was investigated with a modified staining sulforhodamine B assay.The following organic substances were detected: ACP, BHT, BPA, 1,4-BDDMA, CQ, DBP, DMABEE, HEMA, MCE, MeHQ, MOPA, MS, TMPTMA, and TPSb and the ions silicon, aluminum, calcium, sodium, and barium. Activa Bioactive Restorative was found to be biocompatible. Elution of organic substances depended on material’s composition, the nature of the solvent and the storage time. Ions’ release depended on material’s composition and storage time. The newly introduced bioactive restorative was found to be more biocompatible.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26164912