Temporal variations in the gut microbial diversity in response to high-fat diet and exercise

High-fat diet-induced obesity is a pandemic caused by an inactive lifestyle and increased consumption of Western diets and is a major risk factor for diabetes and cardiovascular diseases. In contrast, exercise can positively influence gut microbial diversity and is linked to a decreased inflammatory...

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Published in:Scientific reports 2024-02, Vol.14 (1), p.3282-3282, Article 3282
Main Authors: Imdad, Saba, So, Byunghun, Jang, Junho, Park, Jinhan, Lee, Sam-Jun, Kim, Jin-Hee, Kang, Chounghun
Format: Article
Language:English
Subjects:
631/326
631/326/2565
Animals
Bacteroidetes - classification
Cardiovascular diseases
Community structure
Diabetes mellitus
Diet
Diet, High-Fat - adverse effects
Feces
Gastrointestinal Microbiome
High fat diet
Humanities and Social Sciences
Inflammation
Intestinal microflora
Longitudinal Studies
Mice
Mice, Inbred C57BL
Microbiomes
Microbiota
multidisciplinary
Obesity - etiology
Risk factors
Science
Science (multidisciplinary)
Temporal variations
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Summary:High-fat diet-induced obesity is a pandemic caused by an inactive lifestyle and increased consumption of Western diets and is a major risk factor for diabetes and cardiovascular diseases. In contrast, exercise can positively influence gut microbial diversity and is linked to a decreased inflammatory state. To understand the gut microbial variations associated with exercise and high-fat diet over time, we conducted a longitudinal study to examine the effect of covariates on gut microbial diversity and composition. Young mice were divided into four groups: Chow-diet (CHD), high-fat diet (HFD), high-fat diet + exercise (HFX), and exercise only (EXE) and underwent experimental intervention for 12 weeks. Fecal samples at week 0 and 12 were collected for DNA extraction, followed by 16S library preparation and sequencing. Data were analyzed using QIIME 2, R and MicrobiomeAnalyst. The Bacteroidetes-to-Firmicutes ratio decreased fivefold in the HFD and HFX groups compared to that in the CHD and EXE groups and increased in the EXE group over time. Alpha diversity was significantly increased in the EXE group longitudinally ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-52852-4