Integrative transcriptome analysis reveals alternative polyadenylation potentially contributes to GCRV early infection

IntroductionGrass carp reovirus (GCRV), a member of the Aquareovirus genus in the Reoviridae family, is considered to be the most pathogenic aquareovirus. Productive viral infection requires extensive interactions between viruses and host cells. However, the molecular mechanisms underlying GCRV earl...

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Bibliographic Details
Published in:Frontiers in microbiology 2023-11, Vol.14, p.1269164-1269164
Main Authors: Tan, Sheng, Zhang, Jie, Peng, Yonglin, Du, Wenfei, Yan, Jingxuan, Fang, Qin
Format: Article
Language:English
Subjects:
alternative polyadenylation
Aquareovirus genus
Ctenopharyngodon idellus
DNA methylation
grass carp reovirus
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Summary:IntroductionGrass carp reovirus (GCRV), a member of the Aquareovirus genus in the Reoviridae family, is considered to be the most pathogenic aquareovirus. Productive viral infection requires extensive interactions between viruses and host cells. However, the molecular mechanisms underlying GCRV early infection remains elusive.MethodsIn this study we performed transcriptome and DNA methylome analyses with Ctenopharyngodon idellus kidney (CIK) cells infected with GCRV at 0, 4, and 8 h post infection (hpi), respectively.ResultsWe found that at early infection stage the differentially expressed genes related to defense response and immune response in CIK cells are activated. Although DNA methylation pattern of CIK cells 8 hpi is similar to mock-infected cells, we identified a considerable number of genes that selectively utilize alternative polyadenylation sites. Particularly, we found that biological processes of cytoskeleton organization and regulation of microtubule polymerization are statistically enriched in the genes with altered 3'UTRs.DiscussionOur results suggest that alternative polyadenylation potentially contributes to GCRV early infection.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2023.1269164