Effect of Procyanidin on Canine Sperm Quality during Chilled Storage

• Published in: Veterinary sciences 2022-10, Vol.9 (11), p.588
• Main Authors: Huang, Xiaogang, Zhao, Zhihong, Wang, Ronggen, Ma, Ying, Bu, Yonghui, Hu, Minhua, Zhang, Shouquan
• Format: Article
• Language: English
• Subjects: Antioxidants; Artificial insemination; Biotechnology; canine sperm; Catalase; chilled storage; Dogs; Gene expression; Glutathione peroxidase; Laboratory animals; Lipid peroxidation; Membrane potential; Mitochondria; Motility; Oxidative stress; Pharmaceutical industry; Plasma; procyanidin (PC); Reproductive technologies; Sperm; sperm quality; Superoxide dismutase; Supplements; Vitamin E; Yolk
• ISSN: 2306-7381; 2306-7381
• DOI: 10.3390/vetsci9110588

Procyanidin (PC) is a polyphenolic compound with antioxidant activity. The purpose of this study was to determine the influence of PC on canine sperm quality after 72 h of storage at 4 °C. The collected ejaculates were separated into four equal aliquots and treated with various concentrations of PC (0, 10, 30, and 50 μg/mL) in Tris-citric-fructose-egg yolk (TCFE) extender and stored at 4 °C for 72 h. The findings revealed that 30 μg/mL PC was the optimum concentration for significantly improving sperm motility (p < 0.05). Sperm samples treated with 30 μg/mL PC had substantially greater plasma membrane integrity, acrosome integrity, and mitochondrial membrane potential than the control group (p < 0.05). Furthermore, T-AOC and the expression levels of superoxide dismutase 1 (SOD1), catalase (CAT), and glutathione peroxidase 1 (GPx1) genes were significantly higher in sperm treated with 30 μg/mL PC than those in control (p < 0.05). In summary, this study discovered that adding PC to the TCFE extender enhanced sperm quality and that 30 μg/mL PC was the optimal concentration for canine sperm when stored at 4 °C.