The Efficacy and Biopharmaceutical Properties of a Fixed-Dose Combination of Disulfiram and Benzyl Benzoate

Scabies and hair lice are parasitic diseases that affect human skin and hair, respectively. The incidence and resistances of these infections are increasing. Tenutex® (disulfiram and benzyl benzoate emulsion) is an alternative to standard insecticides to avoid resistances. The aim of the work is to...

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Published in:International journal of molecular sciences 2022-09, Vol.23 (18), p.10969
Main Authors: Lajarin-Reinares, Maria, Martinez-Esteve, Elia, Pena-Rodríguez, Eloy, Cañellas-Santos, Mariona, Bulut, Sanja, Karabelas, Kostas, Clauss, Adam, Nieto, Carles, Mallandrich, Mireia, Fernandez-Campos, Francisco
Format: Article
Language:English
Subjects:
Adults
antiparasitic agents
Benzoates
Benzoic acid
benzyl benzoate
Cytotoxicity
Dermal absorption
Disulfiram
Drug dosages
Drug resistance
Eggs
Explants
Exposure
Hair
Hatchability
High performance liquid chromatography
Infections
Insecticide resistance
Insecticides
Keratinocytes
Lice
Malathion
Parasites
Parasitic diseases
Pediculus humanus capitis
Pharmaceuticals
Psoroptes ovis
Scabies
Skin
skin permeation
topical medication
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Summary:Scabies and hair lice are parasitic diseases that affect human skin and hair, respectively. The incidence and resistances of these infections are increasing. Tenutex® (disulfiram and benzyl benzoate emulsion) is an alternative to standard insecticides to avoid resistances. The aim of the work is to evaluate the transdermal absorption and the in vitro efficacy against scabies and hair lice after different exposition times. Dermatomed human skin was used to assess the dermal absorption using a validated High Performance Liquid Chromatography (HPLC) method. HEK001 keratinocytes were used to evaluate the cytotoxicity of benzyl benzoate. Only benzyl benzoate was able to cross the skin, but it did not show cytotoxicity at any of the tested concentrations. The product efficacy was tested on Psoroptes ovis after direct contact and after administration on sheep skin explants at different contact times. Permethrin/malathion-resistant strains of Pediculus humanis capitis adults and eggs were directly exposed to Tenutex, and the vitality and hatchability, respectively, were evaluated. The anti-scabies study demonstrated that exposure for 6 or 24 h completely eradicated the parasite. The pediculicidal activity of Tenutex exhibited superior efficacy than standard treatment on resistant lice. The positive results obtained suggest that Tenutex® is a good treatment option, especially in drug resistance situations.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms231810969