miR‐99a‐5p modulates doxorubicin resistance via the COX‐2/ABCG2 axis in triple‐negative breast cancer: from the discovery to in vivo studies

• Published in: Cancer communications (London, England) England), 2022-12, Vol.42 (12), p.1412-1416
• Main Authors: Garrido‐Cano, Iris, Adam‐Artigues, Anna, Lameirinhas, Ana, Blandez, Juan F., Candela‐Noguera, Vicente, Rojo, Federico, Zazo, Sandra, Madoz‐Gúrpide, Juan, Lluch, Ana, Bermejo, Begoña, Sancenón, Felix, Cejalvo, Juan Miguel, Martínez‐Máñez, Ramón, Eroles, Pilar
• Format: Article
• Language: English
• Subjects: Apoptosis; ATP Binding Cassette Transporter, Subfamily G, Member 2 - genetics; Breast cancer; Cancer therapies; Consortia; Cyclooxygenase 2; Doxorubicin - pharmacology; Drug resistance; Flow cytometry; Gene expression; Gene Expression Regulation, Neoplastic; Genomes; Humans; Hyaluronic acid; Letter to the Editor; Letters to the Editor; Medical prognosis; MicroRNAs; MicroRNAs - genetics; Microscopy; Nanoparticles; Neoplasm Proteins; Taxonomy; Triple Negative Breast Neoplasms - drug therapy; Triple Negative Breast Neoplasms - genetics
• ISSN: 2523-3548; 2523-3548
• DOI: 10.1002/cac2.12352

Abbreviations ABCG2 ATP-binding cassette subfamily G member 2 ALT alanine transaminase AST aspartate aminotransferase ATCC American Type Culture Collection BC breast cancer BET Brunauer−Emmett−Teller BJH Barret-Joyner-Halenda CI confidence interval COX-2 cyclooxygenase-2 CRE creatinine CTAB Cetyltrimethylammonium bromide DAPI 4',6-diamidino-2-phenylindole DLS dynamic light scattering DRFS distant-relapse free survival FDR false discovery rate FTIR Fourier-transform infrared GEO Gene Expression Omnibus H&E hematoxylin and eosin H&E hematoxylin and eosin HA hyaluronic acid HR hazard ratio IC50 half-maximal inhibitory concentration ICP-MS inductively coupled plasma mass spectrometry METABRIC Molecular Taxonomy of Breast Cancer International Consortium MFI mean fluorescence intensity MSNs mesoporous silica nanoparticles myh7 Myosin Heavy Chain 7 NCBI National Center for Biotechnology Information OS overall survival PEI polyethyleneimine PTFE polytetrafluoroethylene PXRD Powder X-ray diffraction qRT-PCR quantitative real-time PCR RIPA radioimmunoprecipitation RNU43 U43 Small Nuclear RNA SAED selected area electron diffraction SD standard deviation SDS-PAGE sodium dodecyl sulfate–polyacrylamide gel electrophoresis SEM standard error of the mean siRNAs short interfering RNAs TCGA The Cancer Genome Atlas TEM transmission electron microscopy TEOS tetraethylorthosilicate TGA thermogravimetric analysis TMAH tetramethylammonium hydroxide solution TNBC triple-negative breast cancer URE urea UTR untranslated region Dear Editor, Breast cancer (BC) is the most commonly diagnosed cancer and the fifth cause of cancer-related death worldwide [ 1]. In this context, microRNAs have emerged as potential therapeutic targets to overcome therapy resistance [ 4]. [...]we aimed to elucidate the molecular mechanisms underlying resistance to doxorubicin, one of the most effective chemotherapeutic agents used in BC. Additionally, its association with overall survival (OS) was assessed in the Molecular Taxonomy of Breast Cancer International Consortium (METABRIC, n = 1262) and The Cancer Genome Atlas (TCGA) cohorts (n = 1062), which confirmed that low miR-99a-5p expression was associated with poor prognosis (Supplementary Figure S1D-E). [...]we hypothesized that miR-99a-5p could increase doxorubicin sensitivity. miR-99a-5p was described to increase sensitivity to radiation and cisplatin [ 6, 7], but its role in doxorubicin resistance was still unexplored. [...]we hypothesized that ABCG2