Effect of Methionine on Gene Expression in Komagataella phaffii Cells

yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in cells using RN...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-03, Vol.11 (4), p.877
Main Authors: Ianshina, Tatiana, Sidorin, Anton, Petrova, Kristina, Shubert, Maria, Makeeva, Anastasiya, Sambuk, Elena, Govdi, Anastasiya, Rumyantsev, Andrey, Padkina, Marina
Format: Article
Language:English
Subjects:
amino acid metabolism
Amino acids
AOX1 gene
Biosynthesis
Biotechnology
Carbon
Cultivation
Culture media
Dehydrogenases
DNA methylation
E coli
Energy resources
Enzymes
fatty acid metabolism
Fatty acids
Gene expression
Glucose
Glycerol
Homocysteine
Kinases
Komagataella phaffii
Metabolism
Methanol
Methionine
Nitrogen
Optimization
Oxidative stress
Pichia pastoris
Polyamines
Proteins
Sulfur
X-33
Yeast
Yeasts
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Summary:yeast plays a prominent role in modern biotechnology as a recombinant protein producer. For efficient use of this yeast, it is essential to study the effects of different media components on its growth and gene expression. We investigated the effect of methionine on gene expression in cells using RNA-seq analysis. Several gene groups exhibited altered expression when cells were cultured in a medium with methanol and methionine, compared to a medium without this amino acid. Methionine primarily affects the expression of genes involved in its biosynthesis, fatty acid metabolism, and methanol utilization. The gene promoter, which is widely used for heterologous expression in is downregulated in methionine-containing media. Despite great progress in the development of strain engineering techniques, a sensitive adjustment of cultivation conditions is required to achieve a high yield of the target product. The revealed effect of methionine on gene expression is important for optimizing media recipes and cultivation strategies aimed at maximizing the efficiency of recombinant product synthesis.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11040877