Cell and molecular transitions during efficient dedifferentiation

Dedifferentiation is a critical response to tissue damage, yet is not well understood, even at a basic phenomenological level. Developing cells undergo highly efficient dedifferentiation, completed by most cells within 24 hr. We use this rapid response to investigate the control features of dediffer...

Full description

Saved in:
Bibliographic Details
Published in:eLife 2020-04, Vol.9
Main Authors: Nichols, John Me, Antolović, Vlatka, Reich, Jacob D, Brameyer, Sophie, Paschke, Peggy, Chubb, Jonathan R
Format: Article
Language:English
Subjects:
Adaptability
Bacteria
bet-hedging
Biochemistry
Biosynthesis
Cell Dedifferentiation - genetics
cell plasticity
dedifferentiation
Developmental Biology
Dictyostelium
Dictyostelium - cytology
Dictyostelium - physiology
Evolution
Gene Expression
Gene Expression Profiling
Genetic aspects
induced pluripotent stem cells
Kinases
Mutation
Nutrients
Nutrition
reprogramming
Single-Cell Analysis
Stem cells
Stem Cells and Regenerative Medicine
Transcription Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dedifferentiation is a critical response to tissue damage, yet is not well understood, even at a basic phenomenological level. Developing cells undergo highly efficient dedifferentiation, completed by most cells within 24 hr. We use this rapid response to investigate the control features of dedifferentiation, combining single cell imaging with high temporal resolution transcriptomics. Gene expression during dedifferentiation was predominantly a simple reversal of developmental changes, with expression changes not following this pattern primarily associated with ribosome biogenesis. Mutation of genes induced early in dedifferentiation did not strongly perturb the reversal of development. This apparent robustness may arise from adaptability of cells: the relative temporal ordering of cell and molecular events was not absolute, suggesting cell programmes reach the same end using different mechanisms. In addition, although cells start from different fates, they rapidly converged on a single expression trajectory. These regulatory features may contribute to dedifferentiation responses during regeneration.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.55435