Capillary Gas Chromatographic Determination of Gamma Aminobutyric acid and Putrescine in Cerebrospinal Fluid using Trifluoroacetylacetone as Derivatizing Reagent

Abstract In present work, a new capillary gas chromatographic procedure was established and validated for the determination of gamma aminobutyric acid (GABA) and putrescine (Put) using trifluoroacetylacetone (FAA) as derivatizing reagent from Cerebrospinal Fluid (CSF) samples prior to their gas chro...

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Published in:Pakistan journal of analytical and environmental chemistry 2018-06, Vol.19 (1), p.28-35
Main Authors: Zardari, Liaquat Ali, Kamboh, Muhammad Afzal, Nodeh, Hamid Rashidi, Laghari, AbdulJabbar, Khuhawar, Muhammad Yar, Khokhar, Suhail
Format: Article
Language:English
Subjects:
Amino acids
Cerebrospinal Fluid
Chromatography
Detection equipment
Fluids
GABA
Gamma aminobutyric acid
Gas chromatography
Neurosciences
Polyamines
Pump industry
Putrescine
Reagents
Trifluoroacetylacetone
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Summary:Abstract In present work, a new capillary gas chromatographic procedure was established and validated for the determination of gamma aminobutyric acid (GABA) and putrescine (Put) using trifluoroacetylacetone (FAA) as derivatizing reagent from Cerebrospinal Fluid (CSF) samples prior to their gas chromatographic- flame ionization detector (GC-FID) analysis. Up till now various analytical techniques including magnetic resonance spectroscopy [7-9], electrochemical sensor [10, 11], spectrofluorimetry [12], spectrophotometry [13], radio receptor assay [14], capillary electrophoresis (CE) [15-17], liquid chromatography (LC) [18-22] and t gas chromatography (GC), thin layer chromatography [23-28] have been applied for the determination of the GABA, Put and Cad from biological fluids [5, 29-37]. Analysis of GABA and Put from CSF by standard addition method Human cerebrospinal fluids (CSF) (2 mL) were added to methanol (2 mL) and centrifuged at 12000 g for 15 min.The supernatant was alienated in two equal portions and one portion was treated as "GC-FID method". The quantitation was passed out from linear calibration curve and increment in the response for GABA and Put with added amounts. % Recovery of Cad and TA from CSF by standard addition method Human cerebrospinal fluids (CSF) (2 mL) were added to methanol (2 mL) and centrifuged at 12000 g for 15 min.
ISSN:1996-918X
1996-918X
DOI:10.21743/pjaec/2018.06.02