Electron-transfer MALDI MS methodology for microalgae/phytoplankton pigments analysis

•Electron-transfer (ET) reactions effectively ionize pigments in microalgae and phytoplankton extracts.•ET MS affords fast and reliable chemical information for biomarker analysis.•Characteristic spectral regions in MS facilitate data interpretation. The method describes pigment analysis from microa...

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Bibliographic Details
Published in:MethodsX 2023-01, Vol.10, p.102140-102140, Article 102140
Main Authors: Díaz-Sánchez, Luis M., Blanco-Tirado, Cristian, Combariza, Marianny Y.
Format: Article
Language:English
Subjects:
Carotenoids
Chemistry
Chlorophylls
Electron transfer ionization
Electron-transfer MALDI MS methodology for microalgae pigments analysis
MALDI
Mass Spectrometry
Matrix Assisted Laser Desorption Ionization
Microalgae
Photoprotective pigments
Photosynthetic pigments
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Summary:•Electron-transfer (ET) reactions effectively ionize pigments in microalgae and phytoplankton extracts.•ET MS affords fast and reliable chemical information for biomarker analysis.•Characteristic spectral regions in MS facilitate data interpretation. The method describes pigment analysis from microalgae/phytoplankton extracts using electron-transfer Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (ET MALDI MS). Current microalgae/phytoplankton pigment analysis requires resource- and time-intensive chromatographic methods due to the broad polarity range of the target analytes. On the other hand, traditional MALDI MS chlorophyll analysis, using proton-transfer matrices such as 2,5-dihydroxybenzoic acid (DHB) or α-cyano-4-hydroxycinnamic acid (CHCA), results in central metal loss and phytol–ester cleavage. ET MALDI MS is an alternative for the rapid screening and detection of pigments in microalgae extracts.•MALDI matrices with ionization energies above 8.0 eV guarantee electron-transfer processes from photosynthetic and photoprotective pigments whose ionization energies lay below 7.5 eV.•ET MALDI MS pigment analysis agrees with data gathered from conventional chromatographic techniques (HPLC) and optical microscopy for pigment extracts from C. vulgaris cultures and freshwater phytoplankton samples.•The ET MALDI MS method allows fast and reliable detection of pigments in microalgae cultures and freshwater phytoplankton samples. [Display omitted]
ISSN:2215-0161
2215-0161
DOI:10.1016/j.mex.2023.102140