Comparative Genomic Analysis of the Hydrocarbon-Oxidizing Dibenzothiophene-Desulfurizing Gordonia Strains

A number of actinobacteria of the genus are able to use dibenzothiophene (DBT) and its derivatives as the only source of sulfur, which makes them promising agents for the process of oil biodesulfurization. Actinobacteria assimilate sulfur from condensed thiophenes without breaking the carbon-carbon...

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Published in:Microorganisms (Basel) 2022-12, Vol.11 (1), p.4
Main Authors: Frantsuzova, Ekaterina, Delegan, Yanina, Bogun, Alexander, Sokolova, Diyana, Nazina, Tamara
Format: Article
Language:English
Subjects:
Alkanes
Annotations
Aromatic compounds
Asphalt
biodegradation
Bioremediation
Carbon
Covalent bonds
Crude oil
Desulfurization
Desulfurizing
Dibenzothiophene
Diesel fuels
Genes
genome assembly
Genomes
Genomic analysis
Gordonia
hydrocarbon-oxidizing bacteria
Hydrocarbons
Internet service providers
Microorganisms
Oil
Oil pollution
Oil recovery
Oxidation
Paraffin
Plasmids
Software packages
Strains (organisms)
Sulfur
Sulfur content
Surfactants
Thiophenes
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Summary:A number of actinobacteria of the genus are able to use dibenzothiophene (DBT) and its derivatives as the only source of sulfur, which makes them promising agents for the process of oil biodesulfurization. Actinobacteria assimilate sulfur from condensed thiophenes without breaking the carbon-carbon bonds, using the 4S pathway encoded by the dszABC operon-like structure. The genome of the new dibenzothiophene-degrading hydrocarbon-oxidizing bacterial strain 6-1 was completely sequenced and the genes potentially involved in the pathways of DBT desulfurization, oxidation of alkanes and aromatic compounds, as well as in the osmoprotectant metabolism in strain 6-1 and other members of the genus , were analyzed. The genome of . strain 6-1 consists of a 5,105,798-bp circular chromosome (67.3% GC content) and an 86,621-bp circular plasmid, pCP86 (65.4% GC content). This paper presents a comparative bioinformatic analysis of complete genomes of strain 6-1 and dibenzothiophene-degrading strains 1D and 135 that do not have the dsz operon. The assumption is made about the participation in this process of the region containing the B gene. Genomic analysis supported the results of phenomenological studies of strains and the possibility of their application in the bioremediation of oil-contaminated environments and in the purification of oil equipment from oil and asphalt-resin-paraffin deposits.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11010004