Exploring MicroRNAs Associated with Pomegranate Pistil Development: An Identification and Analysis Study

The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differ...

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Published in:Horticulturae 2024-01, Vol.10 (1), p.85
Main Authors: Zhao, Yujie, Huang, Jingyi, Li, Ming, Ren, Hongfang, Jiao, Jian, Wan, Ran, Liu, Yu, Wang, Miaomiao, Shi, Jiangli, Zhang, Kunxi, Hao, Pengbo, Song, Shangwei, Bai, Tuanhui, Zheng, Xianbo
Format: Article
Language:English
Subjects:
Binomial distribution
Cell division
correlation analysis
Data analysis
Developmental stages
Females
Flowers
Genes
Genetic aspects
Genomes
Identification and classification
MicroRNA
MicroRNAs
miRNA
Physiological aspects
pistil
Pistils
Plant growth
Pomegranate
Proteins
Regulation
RNA polymerase
Software
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Summary:The interaction between miRNAs (microRNAs) and target genes plays an important role in plant pistil development. MiRNAs related to pistils were explored in pomegranate. The differentially expressed miRNAs were screened at different developmental stages of pomegranate pistils, and their target differentially expressed mRNAs were further identified to clarify the regulatory effect of miRNAs on pistil development. In our study, 61 conserved miRNAs were identified in 30 families, including miR395, miR394, miR393, miR161, miR162, and miR168. Among them, miR156, miR157, miR159, miR160, miR164, miR165, miR166, miR167, miR169, and miR172 were involved in the development of flower organs. Eight miRNAs were randomly selected and verified for qRT-PCR analysis. The result analysis indicated that miR160, miR164, and miR172 might be positive factors in the regulation of pomegranate pistil development. MiR156 and miR166 might be involved in regulation of pomegranate pistil development as negative factors.
ISSN:2311-7524
2311-7524
DOI:10.3390/horticulturae10010085