Cytokines TNFα, IFNγ and IL-2 Are Responsible for Signal Transmission from the Innate Immunity Protein Tag7 (PGLYRP1) to Cytotoxic Effector Lymphocytes

Studies on the mechanisms of activation of cytotoxic lymphocyte subpopulations are an important research direction in modern immunology. This study provides a detailed analysis of the effect of Tag7 (PGRP-S, PGLYRP1) on the development of lymphocyte subpopulations cytotoxic against MHC-negative tumo...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2020-12, Vol.9 (12), p.2602
Main Authors: Sharapova, Tatiana N, Romanova, Elena A, Ivanova, Olga K, Sashchenko, Lidia P, Yashin, Denis V
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
cancer immunology
CD16 antigen
CD3 antigen
CD4 antigen
CD56 antigen
CD8 antigen
Cell activation
Cytokines
Cytotoxicity
Experiments
Gene expression
Genes
Innate immunity
Interleukin 2
Leukocytes (mononuclear)
Lymphocytes
Major histocompatibility complex
Mammals
Medical research
Monocytes
mRNA
Peripheral blood mononuclear cells
PGLYRP1
programmed cell death
Proteins
Protocol
TREM-1
Tumor cells
Tumor necrosis factor-α
γ-Interferon
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Summary:Studies on the mechanisms of activation of cytotoxic lymphocyte subpopulations are an important research direction in modern immunology. This study provides a detailed analysis of the effect of Tag7 (PGRP-S, PGLYRP1) on the development of lymphocyte subpopulations cytotoxic against MHC-negative tumor cells in a pool of peripheral blood mononuclear cells (PBMCs). The results show that Tag7 can bind to the TREM-1 receptor on the surfaces of monocytes, thereby triggering the expression of mRNA TNFα and IFNγ. The appearance of these cytokines in conditioned medium leads to IL-2 cytokine secretion by CD3 CD4 lymphocytes. In turn, IL-2 facilitates unspecific activation of three cytotoxic cell subpopulations in the PBMC pool: NK (CD16 CD56 ), CD3 CD4 and CD3 CD8 . These subpopulations appear after a certain period of incubation with Tag7 and show toxicity against tumor cells.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells9122602