Challenge infection model for MERS-CoV based on naturally infected camels

• Published in: Virology journal 2020-06, Vol.17 (1), p.1-77, Article 77
• Main Authors: Alharbi, Naif Khalaf, Ibrahim, Osman H, Alhafufi, Ali, Kasem, Samy, Aldowerij, Ali, Albrahim, Raed, Abu-obaidah, Ali, Alkarar, Ali, Bayoumi, Faisal Altaib, Almansour, Ali Mohammed, Aldubaib, Musaad, Al-Abdely, Hail M, Balkhy, Hanan H, Qasim, Ibrahim
• Format: Article
• Language: English
• Subjects: Age; Antibodies; Antiviral agents; Biohazards; Camelidae; Camelus dromedarius; Challenge model; Coronaviruses; Disease control; Disease transmission; Dromedary camels; Farms; Health aspects; Infections; Medical research; MERS-CoV; Middle East respiratory syndrome; Provinces; Respiratory diseases; Saudi Arabia; Serology; Seroprevalence; Studies; Vaccination; Vaccine efficacy; Vaccines; Viral infections
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/s12985-020-01347-5

Middle East Respiratory Syndrome coronavirus (MERS-CoV) is an emerging virus that infects humans and camels with no approved antiviral therapy or vaccine. Some vaccines are in development for camels as a one-health intervention where vaccinating camels is proposed to reduce human viral exposure. This intervention will require an understanding of the prior exposure of camels to the virus and appropriate vaccine efficacy studies in camels. We conducted a cross sectional seroprevalence study in young dromedary camels to determine the rate of MERS-CoV seropositivity in young camels. Next, we utilised naturally infected camels as a natural challenge model that can be used by co-housing these camels with healthy naive camels in a ratio of 1 to 2. This model is aimed to support studies on natural virus transmission as well as evaluating drug and vaccine efficacy. We found that 90% of the screened camels have pre-existing antibodies for MERS-CoV. In addition, the challenge model resulted in MERS-CoV transmission within 48 h with infections that continued for 14 days post challenge. Our finding suggests that the majority of young dromedary camels in Saudi Arabia are seropositive and that naturally infected camels can serve as a challenge model to assess transmission, therapeutics, and vaccine efficacy.