AP2XII-1 and AP2XI-2 Suppress Schizogony Gene Expression in Toxoplasma gondii

is an intracellular parasite that is important in medicine and veterinary science and undergoes distinct developmental transitions in its intermediate and definitive hosts. The switch between stages of is meticulously regulated by a variety of factors. Previous studies have explored the role of the...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-05, Vol.25 (10), p.5527
Main Authors: Jiang, Yucong, Shi, Yuehong, Xue, Yingying, Hu, Dandan, Song, Xingju
Format: Article
Language:English
Subjects:
Animals
ApiAP2
Cell cycle
conditional knockdown
DNA methylation
Epigenetics
Gene expression
Gene Expression Regulation
Genotype & phenotype
Histone Deacetylases - genetics
Histone Deacetylases - metabolism
Infections
merozoite
MORC
Parasites
Proteins
Protozoa
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Toxoplasma - genetics
Toxoplasma - growth & development
Toxoplasma - metabolism
Toxoplasma gondii
Toxoplasmosis - parasitology
Transcription factors
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Summary:is an intracellular parasite that is important in medicine and veterinary science and undergoes distinct developmental transitions in its intermediate and definitive hosts. The switch between stages of is meticulously regulated by a variety of factors. Previous studies have explored the role of the microrchidia (MORC) protein complex as a transcriptional suppressor of sexual commitment. By utilizing immunoprecipitation and mass spectrometry, constituents of this protein complex have been identified, including MORC, Histone Deacetylase 3 (HDAC3), and several ApiAP2 transcription factors. Conditional knockout of MORC or inhibition of HDAC3 results in upregulation of a set of genes associated with schizogony and sexual stages in tachyzoites. Here, our focus extends to two primary ApiAP2s (AP2XII-1 and AP2XI-2), demonstrating their significant impact on the fitness of asexual tachyzoites and their target genes. Notably, the targeted disruption of AP2XII-1 and AP2XI-2 resulted in a profound alteration in merozoite-specific genes targeted by the MORC-HDAC3 complex. Additionally, considerable overlap was observed in downstream gene profiles between AP2XII-1 and AP2XI-2, with AP2XII-1 specifically binding to a subset of ApiAP2 transcription factors, including AP2XI-2. These findings reveal an intricate cascade of ApiAP2 regulatory networks involved in schizogony development, orchestrated by AP2XII-1 and AP2XI-2. This study provides valuable insights into the transcriptional regulation of growth and development, shedding light on the intricate life cycle of this parasitic pathogen.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms25105527