Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus

We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through...

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Bibliographic Details
Published in:Stem cell research 2024-04, Vol.76, p.103332-103332, Article 103332
Main Authors: Orsi, A., Serpieri, V., Mazzotta, C., Ramusino, M. Cotta, Rossi, E., Cerri, S., Valente, E.M.
Format: Article
Language:English
Subjects:
Cell Differentiation
Fibroblasts - metabolism
Humans
Induced Pluripotent Stem Cells - metabolism
Lab Resource: Multiple Cell Lines
Sendai virus - genetics
Online Access:Get full text
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Summary:We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1–60, NANOG and SSEA4) and in vitro differentiation into three germ layers.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2024.103332