Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus

We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through...

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Published in:Stem cell research 2024-04, Vol.76, p.103332-103332, Article 103332
Main Authors: Orsi, A., Serpieri, V., Mazzotta, C., Ramusino, M. Cotta, Rossi, E., Cerri, S., Valente, E.M.
Format: Article
Language:English
Subjects:
Cell Differentiation
Fibroblasts - metabolism
Humans
Induced Pluripotent Stem Cells - metabolism
Lab Resource: Multiple Cell Lines
Sendai virus - genetics
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container_title Stem cell research
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creator Orsi, A.
Serpieri, V.
Mazzotta, C.
Ramusino, M. Cotta
Rossi, E.
Cerri, S.
Valente, E.M.
description We established two iPSC lines starting from skin fibroblasts of two healthy individuals using Sendai-virus-based technique. The obtained iPSCs were characterized showing same STR profile as starting fibroblasts, normal karyotype, loss of stemness vectors, expression of stemness markers, both through real-time PCR and immunofluorescence, (OCT4, SOX2, TRA-1–60, NANOG and SSEA4) and in vitro differentiation into three germ layers.
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subjects Cell Differentiation
Fibroblasts - metabolism
Humans
Induced Pluripotent Stem Cells - metabolism
Lab Resource: Multiple Cell Lines
Sendai virus - genetics
title Generation of iPSC lines derived from skin fibroblasts of two healthy controls using non-transmissible form of Sendai Virus
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