Investigating the Hepatic Response to Orlistat and White Tea in Rats on a High-Fat Diet

• Published in: Life (Basel, Switzerland) Switzerland), 2024-10, Vol.14 (10), p.1283
• Main Authors: Ciftel, Serpil, Klisic, Aleksandra, Ciftel, Enver, Mercantepe, Tolga, Yilmaz, Adnan, Ciftel, Sedat, Pinarbas, Esra, Toraman, Merve Nur, Mercantepe, Filiz
• Format: Article
• Language: English
• Subjects: Antioxidants; Care and treatment; Complications and side effects; Damage prevention; Diet; Erdogan, Recep Tayyip; Fatty liver; FDA approval; Hepatocytes; hepatosteatosis; High fat diet; Homogenization; Inflammation; Ketamine; Laboratory animals; Lipid peroxidation; Lipids; liver; Liver cancer; Liver cirrhosis; Liver diseases; Metabolic disorders; Metabolic syndrome; Metabolism; Nf-kB/p65; NF-κB protein; Obesity; Orlistat; Oxidative stress; Peroxidation; rat; Side effects; Tea; Testing; White tea
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life14101283

High-fat diets have detrimental health impacts that increase the likelihood of developing obesity and metabolic syndrome. This study aimed to examine the potential antioxidant and anti-inflammatory effects of orlistat and white tea in rats fed a high-fat diet. Thirty-two rats were randomized into four groups: control (standard diet), HFD (high-fat diet), HFD+Orlistat (high-fat diet+orlistat), and HFD+WT (high-fat diet+white tea extract). A significant increase in malondialdehyde (MDA) levels and a decrease in total thiol (TT) levels were detected in the HFD group ( < 0.001). On the other hand, a decrease in the MDA level ( < 0.001) and an increase in the TT level were observed in the orlistat and white tea groups compared with those in the HFD group ( < 0.001). Histopathological examinations revealed that, compared with the HFD alone, orlistat and white tea reduced fat accumulation, prevented degenerative changes in hepatocytes, and decreased the histopathological damage score ( = 0.001). Immunohistochemical examinations of nuclear factor-kappa B (NF-kB/p65) revealed that compared with the HFD, orlistat and white tea reduced immunopositivity ( = 0.001). White tea decreases lipid peroxidation and oxidative stress. Both white tea and orlistat decreased fat formation and inflammation in the liver and regulated inflammation by reducing Nf-kB positivity. Nevertheless, further research is needed to assess their impact on human subjects.